The Role of Lactic Acid Bacteria in Milk Fermentation

DOI: 10.4236/fns.2014.54051   PDF   HTML     31,238 Downloads   48,307 Views   Citations

Abstract

Species of lactic acid bacteria (LAB) represent as potential microorganisms and have been widely applied in food fermentation worldwide. Milk fermentation process has been relied on the activity of LAB, where transformation of milk to good quality of fermented milk products made possible. The presence of LAB in milk fermentation can be either as spontaneous or inoculated starter cultures. Both of them are promising cultures to be explored in fermented milk manufacture. LAB have a role in milk fermentation to produce acid which is important as preservative agents and generating flavour of the products. They also produce exopolysaccharides which are essential as texture formation. Considering the existing reports on several health-promoting properties as well as their generally recognized as safe (GRAS) status of LAB, they can be widely used in the developing of new fermented milk products.

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Y. Widyastuti, R.   and A. Febrisiantosa, "The Role of Lactic Acid Bacteria in Milk Fermentation," Food and Nutrition Sciences, Vol. 5 No. 4, 2014, pp. 435-442. doi: 10.4236/fns.2014.54051.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] P. S. Panesar, “Fermented Dairy Products: Starter Cultures and Potential Nutritional Benefits,” Food and Nutrition Sciences, Vol. 2, No. 1, 2011, pp. 47-51.
http://dx.doi.org/10.4236/fns.2011.21006
[2] R. Sharma, B. S. Sanodiya, D. Bagrodia, M. Pandey, A. Sharma and P. S. Bisen, “Efficacy and Potential of Lactic Acid Bacteria Modulating Human Health,” International Journal of Pharma and Bio Sciences, Vol. 3, No. 4, 2012, pp. 935-948.
[3] J. Steele, J. Broadbent and J. Kok, “Perspective on the Contribution of Lactic Acid Bacteria to Cheese Flavor Development,” Current Opinion in Biotechnology, Vol. 24, No. 2, 2013, pp. 135-141.
http://dx.doi.org/10.1016/j.copbio.2012.12.001
[4] S.-N. Liu, Y. Han and Z.-J. Zhou, “Lactic Acid Bacteria in Traditional Fermented Chinese Foods,” Food Research International, Vol. 44, No. 3, 2011, pp. 643-651.
http://dx.doi.org/10.1016/j.foodres.2010.12.034
[5] N. P. Shah, “Functional Cultures and Health Benefits,” International Dairy Journal, Vol. 17, No. 11, 2007, pp. 1262-1277.
http://dx.doi.org/10.1016/j.idairyj.2007.01.014
[6] A. A. Ali, “Beneficial Role of Lactic Acid Bacteria in Food Preservation and Human Health,” Research Journal of Microbiology, Vol. 5, No. 12, 2010, pp. 1213-1221.
http://dx.doi.org/10.3923/jm.2010.1213.1221
[7] M. W. Griffiths and A. M. Tellez, “Lactobacillus helveticus: The Proteolytic System,” Frontiers in Microbiology, Vol. 4, 2013, pp. 1-9.
http://dx.doi.org/10.3389/fmicb.2013.00030
[8] N. F. Olson, “The Impact of Lactic Acid Bacteria on Cheese Flavor,” FEMS Microbiology Reviews, Vol. 87, No. 1-2, 1990, pp. 131-148.
http://dx.doi.org/10.1111/j.1574-6968.1990.tb04884.x
[9] G. Urbach, “Contribution of Lactic Acid Bacteria to Flavour Compound Formation in Dairy Products,” International Dairy Journal, Vol. 5, No. 8, 1995, pp. 877-903.
http://dx.doi.org/10.1016/0958-6946(95)00037-2
[10] P. A. Maragkoudakis, C. Miaris, P. Rojez, N. Manalis, F. Magkanari, G. Kalantzopoulos and E. Tsakalidou, “Production of Traditional Greek Yoghurt Using Lactobacillus Strains with Probiotic Potential as Starter Adjuncts,” International Dairy Journal, Vol. 16, No. 1, 2006, pp. 5260. http://dx.doi.org/10.1016/j.idairyj.2004.12.013
[11] E. Delavenne, J. Mounier, F. Déniel, G. Barbier and G. Le Blay, “Biodiversity of Antifungal Lactic Acid Bacteria Isolated from Raw Milk Samples from Cow, Ewe and Goat over One-Year Period,” International Journal of Food Microbiology, Vol. 155, No. 3, 2012, pp. 185-190.
http://dx.doi.org/10.1016/j.ijfoodmicro.2012.02.003
[12] J. T. M. Wouters, E. H. E. Ayad, J. Hugenholtz and G. Smit, “Microbes from Raw Milk for Fermented Dairy Products,” International Dairy Journal, Vol. 12, No. 2-3, 2002, pp. 91-109.
http://dx.doi.org/10.1016/S0958-6946(01)00151-0
[13] Z. Mennane, K. Khedid, A. Zinedine, M. Lagzouli, M. Ouhssine and M. Elyachioui, “Microbial Characteristics of Klila and Jben Traditionnal Moroccan Cheese from Raw Cow’s Milk,” World Journal of Dairy & Food Sciences, Vol. 2, No. 1, 2007, pp. 23-27.
[14] C. Chaves-López, A. Serio, M. Martuscelli, A. Paparella, E. Osorio-Cadavid and G. Suzzi, “Microbiological Characteristics of Kumis, a Traditional Fermented Colombian Milk, with Particular Emphasis on Enterococci Population,” Food Microbiology, Vol. 28, No. 5, 2011, pp. 1041-1047. http://dx.doi.org/10.1016/j.fm.2011.02.006
[15] M. Ouadghiri, M. Vancanneyt, P. Vandamme, S. Naser, D. Gevers, K. Lefebvre, J. Swings and M. Amar, “Identification of Lactic Acid Bacteria in Moroccan Raw Milk and Traditionally Fermented Skimmed Milk ‘Lben’,” Journal of Applied Microbiology, Vol. 106, No. 2, 2008, pp. 486-495.
http://dx.doi.org/10.1111/j.1365-2672.2008.04016.x
[16] Z. Sun, W. Liu, W. Gao, M. Yang, J. Zhang, L. Wu, J. Wang, B. Menghe, T. Sun and H. Zhang, “Identification and Characterization of the Dominant Lactic Acid Bacteria from Kurut: The Naturally Fermented Yak Milk in Qinghai, China,” Journal of General and Appllied Microbiology, Vol. 56, No. 1, 2010, pp. 1-10.
[17] A. Mayra-Makinen and M. Bigret, “Industrial Use and Production of Lactic Acid Bacteria,” In: S. Salminen, A. von Wright and A. Ouwehand, Eds., Lactic Acid Bacteria Microbiological and Functional Aspects, Marcel Dekker, Inc., New York, 2004, pp. 175-198.
[18] J. M. Kongo, “Lactic Acid Bacteria as Starter-Cultures for Cheese Processing: Past, Present and Future Developments,” Chapter 1, 2013.
http://creativecommons.org/licenses/by/3.0
[19] M. P. Zacharof and R. W. Lovitt, “Bacteriocins Produced by Lactic Acid Bacteria,” APCBEE Procedia, Vol. 2, 2012, pp. 50-56.
http://dx.doi.org/10.1016/j.apcbee.2012.06.010
[20] S.-K. Moon, Y.-J. Wee and G.-W. Choi, “A Novel Lactic Acid Bacterium for the Production of High Purity LLactic Acid, Lactobacillus paracasei subsp. paracasei CHB2121,” Journal of Bioscience and Bioengineering, Vol. 114, No. 2, 2012, pp. 155-159.
http://dx.doi.org/10.1016/j.jbiosc.2012.03.016
[21] Z. Zalán, J. Hudácek, J. Stětina, J. Chumchalová, A. Halász, “Production of Organic Acids by Lactobacillus Strains in Three Different Media,” European Food Research Technology, Vol. 230, No. 3, 2010, pp. 395-404.
http://dx.doi.org/10.1007/s00217-009-1179-9
[22] J. Mufandaedza, B. C. Viljoen, S. B. Feresu and T. H. Gadaga, “Antimicrobial Properties of Lactic Acid Bacteria and Yeast-LAB Cultures Isolated from Traditional Fermented Milk against Pathogenic Escherichia coli and Salmonella enteritidis Strains,” International Journal of Food Microbiology, Vol. 108, No. 1, 2006, pp. 147-152.
http://dx.doi.org/10.1016/j.ijfoodmicro.2005.11.005
[23] H. Li, L. Liu, S. Zhang, H. Uluko, W. Cui and J. Lv, “Potential Use of Lactobacillus casei AST18 as a Bioprotective Culture in Yogurt,” Food Control, Vol. 34, No. 2, 2013, pp. 675-680.
http://dx.doi.org/10.1016/j.foodcont.2013.06.023
[24] E. M. Balciunas, F. A. C. Martinez, S. D. Todorov, B. D. G. de Melo Franco, A. Converti and R. P. de Souza Oliveira, “Novel Biotechnological Applications of Bacteriocins: A Review,” Food Control, Vol. 32, No. 1, 2013, pp. 134-142.
http://dx.doi.org/10.1016/j.foodcont.2012.11.025
[25] S. Mitra, P. K. Chakrabartty and S. R. Biswas, “Potential Production and Preservation of Dahi by Lactococcus lactis W8 a Nisin-Producing Strain,” LWT-Food Science and Technology, Vol. 43, No. 2, 2010, pp. 337-342.
http://dx.doi.org/10.1016/j.lwt.2009.08.013
[26] á. Alegría, S. Delgado, C. Roces, B. López and B. Mayo, “Bacteriocins Produced by Wild Lactococcus lactis Strains Isolated from Traditional Starter-Free Cheeses Made of Raw Milk,” International Journal of Food Microbiology, Vol. 143, No. 1, 2010, pp. 61-66.
http://dx.doi.org/10.1016/j.ijfoodmicro.2010.07.029
[27] S. Mitra, P. K. Chakrabartty and S. R. Biswas, “Potential Production and Preservation of Dahi by Lactococcus lactis W8, a Nisin-Producing Strain,” LWT-Food Science and Technology, Vol. 43, No. 2, 2010, pp. 337-342.
http://dx.doi.org/10.1016/j.lwt.2009.08.013
[28] B. Kumar, P. P. Balgir, B. Kaur and N.Garg, “Cloning and Expression of Bacteriocins of Pediococcus spp.: A Review,” Archives of Clinical Microbiology, Vol. 2, No. 3, 2011, p. 4.
[29] M. Yvon and L. Rijnen, “Cheese Flavour Formation by Amino Acid Catabolism,” International Dairy Journal, Vol. 11, No. 4-7, 2001, pp. 185-201.
http://dx.doi.org/10.1016/S0958-6946(01)00049-8
[30] P. L. H. McSweeney and M. J. Sousa, “Biochemical Pathways for the Production of Flavour Compounds in Cheese during Ripening: A Review,” Lait, Vol. 80, No. 3, 2000, pp. 293-324. http://dx.doi.org/10.1051/lait:2000127
[31] P. Hols, F. Hancy, L. Fontaine, B. Grossiord, D. Prozzi, N. Leblond-Bourget, B. Decaris, A. Bolotin, C. Delorme, S. D. Ehrlich, E. Guédon, V. Monnet, P. Renault and M. Kleerebezem, “New Insights in the Molecular Biology and Physiology of Streptococcus thermophilus Revealed by Comparative Genomics,” FEMS Microbiology Review, Vol. 29, No. 3, 2005, pp. 435-463.
[32] J. R. Broadbent, H. Cai, R. L. Larsen, J. E. Hughes, D. L. Welker, V. G. De Carvalho, T. A. Tompkins, Y. Ardo, F. Vogensen, A. De Lorentiis, M. Gatti, E. Neviani and J. L. Steele, “Genetic Diversity in Proteolytic Enzymes and Amino Acid Metabolism among Lactobacillus helveticus Strains,” Journal of Dairy Science, Vol. 94, No. 9, 2011, pp. 4313-4328. http://dx.doi.org/10.3168/jds.2010-4068
[33] J. Steele, J. Broadbent and J. Kok, “Perspective on the Contribution of Lactic Acid Bacteria to Cheese Flavor Development,” Current Opinion in Biotechnology, Vol. 24, No. 2, 2013, pp. 135-141.
http://dx.doi.org/10.1016/j.copbio.2012.12.001
[34] W. Routray and H. N. Mishra, “Scientific and Technical Aspects of Yogurt Aroma and Taste: A Review,” Comprehensive Reviews in Food Science and Food Safety, Vol. 10, No. 4, 2011, pp. 208-220.
http://dx.doi.org/10.1111/j.1541-4337.2011.00151.x
[35] P. F. Fox, P. L. H. McSweeney, T. M. Cogan and T. P. Guinee, “Cheese: Chemistry, Physics and Microbiology,” Elsevier, 2004.
[36] B. Dias and B. Weimer, “Conversion of Methionine to Thiols by Lactococci, Lactobacilli, and Brevibacteria,” Applied and Environmental Microbiology, Vol. 64, No. 9, 1998, pp. 3320-3326.
[37] J. A. Hannon, K. N. Kilcawley, M. G. Wilkinson, C. M. Delahunty and T. P. Beresford, “Flavor Precursor Development in Cheddar Cheese Due to Lactococcal Starters and the Presence and Lysis of Lactobacillus helveticus,” International Dairy Journal, Vol. 17, No. 4, 2007, pp. 316327.
[38] S. Helinck, D. L. Bars, D. Moreau and M. Yvon, “Ability of Thermophilic Lactic Acid Bacteria to Produce Aroma Compounds from Amino Acids,” Applied and Environmental Microbiology, Vol. 70, No. 7, 2004, pp. 38553681.
http://dx.doi.org/10.1128/AEM.70.7.3855-3861.2004
[39] N. Klein, M. B. Maillard, A. Thierry and S. Lortal, “Conversion of Amino Acids into Aroma Compounds by CellFree Extracts of Lactobacillus helveticus,” Journal of Applied Microbiology, Vol. 91, No. 3, 2001, pp. 404-411.
http://dx.doi.org/10.1046/j.1365-2672.2001.01391.x
[40] O. Kenny, R. J. FitzGerald, G. O’Cuinn, T. Beresford and K. Jordan, “Autolysis of Selected Lactobacillus helveticus Adjunct Strains during Cheddar Cheese Ripening,” International Dairy Journal, Vol. 16, No. 7, 2006, pp. 797-804.
http://dx.doi.org/10.1016/j.idairyj.2005.07.008
[41] W. J. Lee, D. S. Banavara, J. E. Hughes, J. K. Christiansen, J. L. Steele, J. R. Broadbent and S. A. Rankin, “Role of Cystathionine β-Lyase in Catabolism of Amino Acids to Sulfur Volatiles by Genetic Variants of Lactobacillus helveticus CNRZ 32,” Applied and Environmental Microbiology, Vol. 73, No. 9, 2007, pp. 3034-3039.
http://dx.doi.org/10.1128/AEM.02290-06
[42] J. K. Christiansen, J. E. Hughes, D. L. Welker, B. T. Rodríguez, J. L. Steele and J. R. Broadbent, “Phenotypic and Genotypic Analysis of Amino Acid Auxotrophy in Lactobacillus helveticus CNRZ 32,” Applied and Environmental Microbiology, Vol. 74, No. 2, 2008, pp. 416-423.
http://dx.doi.org/10.1128/AEM.01174-07
[43] M. Callanan, P. Kaleta, J. O’Callaghan, O. O’Sullivan, K. Jordan, O. McAuliffe, A. Sangrador-Vegas, L. Slattery, G. F. Fitgerald, T. Beresford and R. P. Ross, “Genome Sequence of Lactobacillus helveticus, an Organism Distinguished by Selective Gene Loss and Insertion Sequence Element Expansion,” Journal of Bacteriology, Vol. 190, No. 2, 2008, pp. 727-735.
http://dx.doi.org/10.1128/JB.01295-07
[44] L. Slaterry, J. O. Callaghan, G. F. Fitgerald, T. Beresford and R. P. Ross, “Invited Review: Lactobacillus helveticusA Thermophilic Dairy Starter Related to Gut Bacteria,” Journal of Dairy Science, Vol. 93, No. 10, 2010, pp. 44354454. http://dx.doi.org/10.3168/jds.2010-3327
[45] M. Gobbetti, M. De Angelis, R. Di Cagno and C. G. Rizzello, “The Relative Contributions of Starter Cultures and Non-Starter Bacteria to the Flavour of Cheese,” In: B. C. Weimer, Ed., Improving the Flavor of Cheese, CRC Press Boca Raton, 2007, pp. 121-156.
[46] M. Gatti, C. Lazzi, L. Rossetti, G. Mucchetti and E. Neviani, “Biodiversity in Lactobacillus helveticus Strains Present in Natural Whey Starter Used for Parmigiano Reggiano Cheese,” Journal of Applied Microbiology, Vol. 95, No. 3, 2003, pp. 463-470.
http://dx.doi.org/10.1046/j.1365-2672.2003.01997.x
[47] L. Rossetti, M. E. Fornasari, M. Gatti, C. Lazzi, E. Neviani and G. Giraffa, “Grana Padano Cheese Whey Starters: Microbial Composition and Strain Distribution,” International Journal of Food Microbiology, Vol. 127, No. 1-2, 2008, pp. 168-171.
http://dx.doi.org/10.1016/j.ijfoodmicro.2008.06.005
[48] L. Fernández, T. Bhowmik and J. Steele, “Characterization of the Lactobacillus helveticus CNRZ32 pepC Gene,” Applied and Environmental Microbiology, Vol. 60, No. 1, 1994, pp. 333-336.
[49] E. Soeryapranata, J. R. Powers and G. ünlü, “Cloning and Characterization of Debittering Peptidases, PepE, PepO, PepO2, PepO3 and PepN, of Lactobacillus helveticus WSU 19,” International Dairy Journal, Vol. 17, No. 9, 2007, pp. 1096-1106.
[50] L. Settani and G. Moschetti., “Non-Starter Lactic Acid Bacteria Used to Improve Cheese Quality and Provide Health Benefits,” Food Microbiology, Vol. 27, No. 6, 2010, pp. 691-697.
http://dx.doi.org/10.1016/j.fm.2010.05.023
[51] T. P. Beresford, N. A. Fitzsimons, N. L. Brennan and T. M. Cogan, “Recent Advances in Cheese Microbiology,” International Dairy Journal, Vol. 11, No. 4-7, 2001, pp. 259-274.
http://dx.doi.org/10.1016/S0958-6946(01)00056-5
[52] T. X. Yang, K. Y. Wu, F. Wang, X. L. Liang, Q. S. Liu, G. Li and Q. Y. Li, “Effect of Exopolysaccharides from Lactic Acid Bacteria on the Texture and Microstructure of Buffalo Yoghurt,” International Dairy Journal, Vol. 34, No. 2, 2014, pp. 252-256.
http://dx.doi.org/10.1016/j.idairyj.2013.08.007
[53] J. Fioramonti, V. Theodorou and L. Bueno, “Probiotics: What Are They? What Are Their Effects on Gut Physiology?” Best Practice & Research Clinical Gastroenterology, Vol. 17, No. 5, 2003, pp. 711-724.
http://dx.doi.org/10.1016/S1521-6918(03)00075-1
[54] I. P. Kaur, K. Chopra and A. Saini, “Probiotics: Potential Pharmaceutical Applications,” European Journal of Pharmaceutical Sciences, Vol. 15, No. 1, 2002, pp. 1-9.
http://dx.doi.org/10.1016/S0928-0987(01)00209-3
[55] T. Vasiljevic and N. P. Shah, “Probiotics—From Metchnikoff to Bioactives,” International Dairy Journal, Vol. 18, No. 7, 2008, pp. 714-728.
http://dx.doi.org/10.1016/j.idairyj.2008.03.004
[56] G. Giraffa, N. Chanishvili and Y. Widyastuti, “Importance of Lactobacilli in Food and Feed Biotechnology,” Research in Microbiology, Vol. 161, No. 6, 2010, pp. 480487.
http://dx.doi.org/10.1016/j.resmic.2010.03.001
[57] K. Singh, B. Kallali, A. Kumar and V. Thaker, “Probiotics: A Review,” Asian Pacific Journal of Tropical Biomedicine, Vol. 1, No. 2, 2011, pp. S287-S290.
[58] J. Hemsworth, S. Hekmat and G. Reid., “The Development of Micronutrient Supplemented Probiotic Yogurt for People Living with HIV: Laboratory Testing and Sensory Evaluation,” Innovative Food Science and Emerging Technologies, Vol. 12, No. 1, 2011, pp. 79-84.
http://dx.doi.org/10.1016/j.ifset.2010.11.004
[59] Z. Guler and A. C. Gürsoy-Balci, “Evaluation of Volatile Compounds and Free Fatty Acids in Set Types Yogurts Made of Ewes’, Goats’ Milk and Their Mixture Using Two Different Commercial Starter Cultures during Refrigerated Storage,” Food Chemistry, Vol. 127, No. 3, 2011, pp. 1065-1071.
http://dx.doi.org/10.1016/j.foodchem.2011.01.090
[60] F. Altay, F. Karbancioglu-Güler, C. Daskaya-Dikmen and D. Heperkan, “A Review on Traditional Turkish Fermented Non-Alcoholic Beverages: Microbiota, Fermentation Process and Quality Characteristics,” International Journal of Food Microbiology, Vol. 167, No. 1, 2013, pp. 44-56.
http://dx.doi.org/10.1016/j.ijfoodmicro.2013.06.016
[61] S. Ortu, G. E. Felis, M. Marzotto, A. Deriu, P. Molicotti, L. A. Sechi, F. Dellaglio and S. Zanetti, “Identification and Functional Characterization of Lactobacillus Strains Isolated from Milk and Gioddu, a Traditional Sardinian Fermented Milk,” International Dairy Journal, Vol. 17, No. 11, 2007, pp. 1312-1320.
http://dx.doi.org/10.1016/j.idairyj.2007.02.008
[62] W. Liu, Q. Bao, Jirimutu, M. Qing, Siriguleng, X. Chen, T. Sun, M. Li, J. C. Zhang, M. Bilige, T. S. Sun and H. P. Zhang, “Isolation and Identification of Lactic Acid Bacteria from Tarag in Eastern Inner Mongolia of China by 16S rRNA Sequences and DGGE Analysis,” Microbiological Research, Vol. 167, No. 2, 2012, pp. 110-115.
http://dx.doi.org/10.1016/j.micres.2011.05.001
[63] T. Matsuzaki, M. Saito, K. Usuku, H. Nose, S. Izumo, K. Arimura and M. Osame, “A Prospective Uncontrolled Trial of Fermented Milk Drink Containing Viable Lactobacillus casei Strain Shirota in the Treatment of HTLV-1 Associated Myelopathy/Tropical Spastic Paraparesis,” Journal of the Neurological Sciences, Vol. 237, No. 1, 2005, pp. 75-81.
http://dx.doi.org/10.1016/j.jns.2005.05.011
[64] R. D. Cagno, A. Tamborrino, G. Gallo, C. Leone, M. De Angelis, M. Faccia, P. Amiranteb and M. Gobbettia, “Uses of Mares’ Milk in Manufacture of Fermented Milks,” International Dairy Journal, Vol. 14, No. 9, 2004, pp. 767-775. http://dx.doi.org/10.1016/j.idairyj.2004.02.005
[65] M. Ouadghiri, M. Vancanneyt, P. Vandamme, S. Naser, D. Gevers, K. Lefebvre, J. Swings and M. Amar, “Identification of Lactic Acid Bacteria in Moroccan Raw Milk and Traditionally Fermented Skimmed Milk ‘Lben’,” Journal of Applied Microbiology, Vol. 106, No. 2, 2008, pp. 486-495.
http://dx.doi.org/10.1111/j.1365-2672.2008.04016.x
[66] F. Nejati, C. G. Rizzello, R. Di Cagno, M. Sheikh-Zeinoddin, A. Diviccaro, F. Minervini and M. Gobbetti, “Manufacture of a Functional Fermented Milk Enriched of Angiotensin-I Converting Enzyme (ACE)-Inhibitory Peptides and g-Amino Butyric Acid (GABA),” LWT-Food Science and Technology, Vol. 51, No. 1, 2013, pp. 183-189.
http://dx.doi.org/10.1016/j.lwt.2012.09.017
[67] C. Chaves-López, A. Serio, M. Martuscelli, A. Paparella, E. Osorio-Cadavid and G. Suzzi, “Microbiological Characteristics of Kumis, a Traditional Fermented Colombian Milk, with Particular Emphasis on Enterococci Population,” Food Microbiology, Vol. 28, No. 5, 2011, pp. 10411047. http://dx.doi.org/10.1016/j.fm.2011.02.006
[68] M. Iranmesh, H. Ezzatpanah and N. Mojgani, “Antibacterial Activity and Cholesterol Assimilation of Lactic Acid Bacteria Isolated from Traditional Iranian Dairy Products,” LWT-Food Science and Technology, 2013, pp. 1-5.
[69] J. M. Mathara, U. Schillinger, P. M. Kutima, S. K. Mbugua and W. H. Holzapfel, “Isolation, Identification and Characterisation of the Dominant Microorganisms of Kule Naoto: The Maasai Traditional Fermented Milk in Kenya,” International Journal of Food Microbiology, Vol. 94, No. 3, 2004, pp. 269-278.
http://dx.doi.org/10.1016/j.ijfoodmicro.2004.01.008
[70] T. A. Lore, S. K. Mbugua and J. Wangoh, “Enumeration and Identification of Icroflora in Suusac, a Kenyan Traditional Fermented Camel Milk Product,” LWT-Food Science and Technology, Vol. 38, No. 2, 2005, pp. 125130. http://dx.doi.org/10.1016/j.lwt.2004.05.008

  
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