Comparison of Different Washing and Disinfection Protocols Used by Food Services in Southern Brazil for Lettuce (Lactuca sativa)

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DOI: 10.4236/fns.2012.31006   PDF   HTML     6,995 Downloads   10,674 Views   Citations

Abstract

Different protocols have been used for washing and disinfecting leafy vegetables in Brazilian food services, however its effect on microbial reduction was barely studied. The aim of this study was to evaluate the effectiveness of lettuce washing and disinfecting protocols used by food services. The treatments evaluated were: washing in potable tap water (T1); washing and dipping in potable tap water (T2); washing with potable tap water and dipping in a solution of sodium hypochlorite (200 ppm of free chlorine) for 15 min (T3); and 30 min (T4); washing with potable tap water and dipping in 2% vinegar solution (T5); and 20% vinegar solution (T6). After the treatments, the samples were microbiologically evaluated to measure bacterial reductions. The microbial counts showed that the mean total mesophile reductions were: 0.64 log for T1, 0.75 log for T2, 2.06 log for T3, 2.46 log for T4, 1.68 log for T5 and 1.72 log for T6, and the mean total coliform reductions were: 1.11 log for T1, 1.09 log for T2, 2.29 log for T3, 2.35 log for T4, 1.6 log for T5 and 2.09 log for T6. Based on these results it can be concluded that protocols which used 200 ppm sodium hypochlorite for 15 to 30 min (T3 e T4), were the most effective, followed by treatment with 20% vinegar solution (T6). The latter method, however, turned the lettuce dark during the distribution period.

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A. Oliveira, A. Ritter, E. Tondo and M. Cardoso, "Comparison of Different Washing and Disinfection Protocols Used by Food Services in Southern Brazil for Lettuce (Lactuca sativa)," Food and Nutrition Sciences, Vol. 3 No. 1, 2012, pp. 28-33. doi: 10.4236/fns.2012.31006.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] S. T. Philippi, “Nutri??o e Técnica Dietética,” Manole, Barueri, 2003.
[2] L. R. R. Santana, R. D. S. Carvalho, C. C. Leite, L. M. Alcantara, T. W. S. Oliveira and B. M. Rodrigues, “Qualidade Fisica, Microbiológica e Parasitológica de Alfaces (Lactuca sativa) de Diferentes Sistemas de Cultivo,” Ciência e Tecnologia de Alimentos, Vol. 26, No. 2, 2006, pp. 264-269. doi:10.1590/S0101-20612006000200006
[3] M. Abadias, J. Usall, M. Anguera, C. Solsona and I. Vi?as, “Microbiological Quality of Fresh, Minimally-Processed Fruit and Vegetables, and Sprouts from Retail Establishments,” International Journal Food Microbiology, Vol. 123, No. 1-2, 2008, pp. 121-129. doi:10.1016/j.ijfoodmicro.2007.12.013
[4] N. Holden, L. Pritchard and I. Toth, “Colonization out-with the Colon: Plants as an Alternative Environmental Reservoir for Human Pathogenic Enterobacteria,” FEMS Microbiology Reviews, Vol. 33, No. 4, 2009, pp. 689-703. doi:10.1111/j.1574-6976.2008.00153.x
[5] J. P. Nourti, T. Niskanen, S. Hallanvuo, J. Mikkola, E. Kela, M. Hatakka, M. Fredriksson-Ahomaa, O. Lyytik?inen, A.Siitonen, H. Korkeala and P. Ruutu, “A Widespread Outbreak of Yersinia pseudotuberculosis O:3 Infection from Iceberg Lettuce,” Journal Infectious Deseas Vol. 189, No. 5, 2004, pp.766-774. doi:10.1086/381766
[6] S. R. P. Silva, S. E. F. Verdin, D. C. Pereira, A. M. Schatkoski, M. B.Rott and G. Cor??o, “Microbiological Quality of Minimally Processed Vegetables Sold in Porto Alegre, Brazil,” Brazilian Journal of Microbiology, Vol. 38, No. 4, 2007, pp. 594-598. doi:10.1590/S1517-83822007000400003
[7] E. D. Hilborn, J. H. Mermin, P. A. Mshar, J. L. Hadler, A. Voetsch, C. Wojtkunski, M. Swartz, R. Mshar, M. A. Lambert-Fair, J. A. Farrar, M. K. Glynn and L. Slutsker, “Multistate Outbreak of Escherichia coli 0157:H7 Infections Associated with Consumption of Mesclum Lettuce,” Archives of Internal Medicine, Vol. 159, No. 15, 1999, pp. 1758-1764. doi:10.1001/archinte.159.15.1758
[8] G. Kapperud, L. M. Rorvik, V. Hasseltvedt, E. A. Hoiby, B. G. Iversen, K. Staveland, G. Johnsen, J. Leitao, H. Herikstad, Y. Andersson, G. Langeland, B. Gondrosen and J. Lassen, “Outbreak of Shigella sonnei Infection Traced to Imported Iceberg Lettuce,” Journal of Clinical Microbiology, Vol. 33, No. 3, 1995, pp. 609-614.
[9] M. S. Nascimento, N. Silva, M. P. L. Catanozi and K. C. Silva, “Avalia??o Comparativa de Diferentes Desinfetantes na Sanitiza??o de uva,” Brazilian Journal Food Technology, Vol. 6, 2003, pp. 63-68.
[10] S. Rubino, P. Cappuccinelli and D. J. Kelvin, “Escherichia coli (STEC) Serotype O104 Outbreak Causing Haemolytic Syndrome (HUS) in Germany and France,” The Journal Infection Developing Countries, Vol. 5, No. 6, 2011, pp. 437-440.
[11] World Health Organization Regional Office for Europe, 2011. http://www.euro.who.int/en/what-we-do/health-topics/emergecies/international-health-regulations/news/news/2011/ 06/ehec-outbreak-update-22
[12] M. Pirovani, A. Piagentini, D. Güemes and S. Arkwright, “Reduction of Chlorine Concentration and Microbial Load during Washing-Disinfection of Shredded Lettuce,” International Journal of Food Science & Technology, Vol. 39, No. 3, 2004, pp. 341-347. doi:10.1111/j.1365-2621.2004.00791.x
[13] Brasil, Ministério da Saúde, Agência Nacional de Vigi- lancia Sanitária, Resolu??o—RDC No. 216 de 15 de Setembro de 2004. http://www.anvisa.gov.br
[14] M. S. Nascimento, N. Silva, M. P. L. M. Catanozi and K. C. Silva, “Effects of Different Disinfectition Treatments on Natural Microbiota of Lettuce,” Journal Food Protection, Vol. 66, No 9, 2003, pp. 1697-1700.
[15] Rio Grande do Sul, Secretaria da Saúde, Portaria No. 78/ 2009. http://www.saude.rs.gov.br
[16] N. Silva, V. C. A. Junqueira and N. F. A. Silveira, “Manual de Métodos de Análise Microbiológica de Alimentos,” Varela, S?o Paulo, 1997.
[17] SPSS—Statistical Package for the Social Sciences (Computer program), Version 8.0, McGraw-Hill Book Company, Chicago, 1998.
[18] Brasil, Ministério da Saúde, Agência Nacional de Vigilancia Sanitária, Resolu??o—RDC No. 12, de 02 de Janeiro de 2001. http://www.anvisa.gov.br
[19] Mercosul/GMC, Grupo Mercado Comum, Resolu??o No. 28 de 20 de Junho de 2002. http://www.anvisa.gov.br
[20] A. Allende, E. Aguayo and F. Artés, “Microbial and Sensory Quality of Commercial Fresh Processed Red Lettuce throughout the Production Chain and Shelf Life,” International Journal Microbiology, Vol. 91, No. 2, 2004, pp. 109-117. doi:10.1016/S0168-1605(03)00373-8
[21] L. A. Keskinen, A. Burke and B. A. Annous, “Efficacy of Chlorine, Acidic Electrolysed Water and Aqueous Chlorine Dioxide Solutions to Decontaminate Escherichia coli O157:H7 from Lettuce Leaves,” International Journal Food Microbiology, Vol. 132, No. 2-3, 2009, pp. 134-140.
[22] F. López-Galvez, A. Allende, M. V. Selma and M. I. Gil, “Prevention of Escherichia coli Cross-Contamination by Different Commercial Sanitizers during Washing of Fresh-Cut Lettuce,” International Journal Food Microbiology, Vol. 133, No. 1-2, 2009, pp. 167-171. doi:10.1016/j.ijfoodmicro.2009.05.017

  
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