TITLE:
Antimicrobial Resistance and Plasmid Profiles of Salmonella enterica Serovars from Different Sources in Lagos, Nigeria
AUTHORS:
K. O. Akinyemi, S. O. Ajoseh, B. A. Iwalokun, A. O. B. Oyefolu, C. O. Fakorede, R. O. Abegunrin, O. Adunmo
KEYWORDS:
Salmonella, Resistant, Antibiogram, Plasmid, Epidemiology, Disease
JOURNAL NAME:
Health,
Vol.10 No.6,
June
22,
2018
ABSTRACT: Introduction: Salmonella
enterica Serovars remains one of
the leading pathogens that cause diarrhoea and bloodstream infections in
developing countries. The emergence of multidrug resistant (MDR) Salmonella has
become a serious problem globally. This study investigated the antibiotic
resistance and plasmid profiles of Salmonella isolates from different
sources. Methods: Seventy-three samples comprised of clinical (30), hand
swab (15), food (10) and water (18) were analyzed bacteriologically. Salmonella isolates were identified and characterized by standard procedures. Isolates
were subjected to antimicrobial susceptibility testing and were further
screened for plasmid DNA by standard methods. Results: A total of 27 Salmonella isolates made up of 5 (18.5%) S.
typhi, 6 (22.2%) S. enteritidis,
9 (33.3) S. typhimurium, 5
(18.5%) S. cholerasuis, and 1 (3.7%)
each of S.arizonae and S. vichow were obtained in this study. All the isolates developed resistance to three
or more antibiotics evaluated. Four distinct resistance profiles: TetAmpCol, TetAmpColCot,
TetAmpColCip and TetAmpColCotCip were recorded with 63% of the isolates
exhibiting resistance profile TetAmpColCot. Specifically 23 of 27 (85.2%) of
the isolates harboured plasmid DNA comprised of 12 distinct plasmid profiles
of different sizes ranging from 3.2 kb to 30.2kb. Salmonella isolates
of the same species from different sources differed in plasmid profile.
Plasmid profile was found to show good discriminatory capability compared
to antibiotics resistance profile. Conclusion: This study revealed that
both resistance antibiogram and plasmid profile are still viable
epidemiological tools for tracing the source of Salmonella isolates. A
need for prudent use of antibiotics is suggested.