TITLE:
Comparison between different methods of estimation of vitamin D
AUTHORS:
Mrinal Pal, Subinay Datta, Amit Kumar Pradhan, Lekha Biswas, Joydeep Ghosh, Partha Mondal, Rajarshi Rahut, Arghya Ray Chaudhuri, Sourav Sau, Soumi Das
KEYWORDS:
Vitamin D; High-Pressure Liquid Chromatography; Chemiluminescent Immunoassays; Enzyme Immunoassays
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.3 No.5,
October
24,
2013
ABSTRACT: Background: Vitamin D is not only important for bone health but can also affect the development of several non-bone diseases. These findings have increased the need for determining vitamin D status in a convenient and cost-effective way. In order to determine the better method for detecting vitamin D status, we have estimated blood vitamin D in three methods such as high-pressure liquid chromatography, chemiluminescent immunoassays and enzyme immunoassays. Method: Two hundred and sixteen subjects irrespective of age and sex were studied for blood vitamin D in all the three methods over a period of 2 years. Result: The statistical analysis showed that values of vitamin D obtained by chemiluminescent immunoassays (r = 0.978, p