Comparison between different methods of estimation of vitamin D


Background: Vitamin D is not only important for bone health but can also affect the development of several non-bone diseases. These findings have increased the need for determining vitamin D status in a convenient and cost-effective way. In order to determine the better method for detecting vitamin D status, we have estimated blood vitamin D in three methods such as high-pressure liquid chromatography, chemiluminescent immunoassays and enzyme immunoassays. Method: Two hundred and sixteen subjects irrespective of age and sex were studied for blood vitamin D in all the three methods over a period of 2 years. Result: The statistical analysis showed that values of vitamin D obtained by chemiluminescent immunoassays (r = 0.978, p < 0.001) were more significantly correlated with high-pressure liquid chromatography than enzyme immunoassays (r = 0.970, p < 0.001). Conclusion: We conclude that chemiluminescent immunoassays should be used instead of enzyme immunoassay and high-pressure liquid chromatography for estimation of vitamin D.

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Pal, M. , Datta, S. , Pradhan, A. , Biswas, L. , Ghosh, J. , Mondal, P. , Rahut, R. , Chaudhuri, A. , Sau, S. and Das, S. (2013) Comparison between different methods of estimation of vitamin D. Advances in Biological Chemistry, 3, 501-504. doi: 10.4236/abc.2013.35054.

Conflicts of Interest

The authors declare no conflicts of interest.


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