TITLE:
PCR-based molecular markers linked to the leaf rust resistance gene Lr19 in different bread wheat cultivars
AUTHORS:
I. M. Huseynova, F. B. Guliyeva, S. M. Rustamova, Jalal A. Aliyev
KEYWORDS:
Wheat; Brown Leaf Rust; Lr19 Gene; SCAR-Markers; PCR Analysis
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.3 No.1,
February
26,
2013
ABSTRACT:
61 varieties of wheat collected in the gene fund of
the Research Institute of Crop Husbandry were screened using SCAR-markers
associated with the gene of resistance to brown leaf rust, Lr19. As a result
of PCR analysis using SCS123 marker the 737 bp locus was detected in 48
genotypes. The expected fragment of the 688 bp was detected in 53 genotypes
using the SCS253 marker. The results obtained using both markers indicate that
the Lr19 gene is present on 7D chromosomes of 45 genotypes. The existence of
the Lr19 gene has not been proven only for 5 from the 61 analyzed wheat
genotypes.