C. Ito, Y. Thoyama, M. Omura, I. Kajira and H. Furukawa, “Alkaloidal Constituents of Murraya koengii. Isolation and Structural Elucidation of Novel Binary Carbazolequinones and Carbazole Alkaloids,” Chemical and Pharmaceutical Bulletin, Vol. 41, No. 12, 1993, pp. 2096-2100. doi:10.1248/cpb.41.2096

has been cited by the following article:

• TITLE: Anti-Inflammatory Effect of 3-Methylcarbazoles on RAW 264.7 Cells Stimulated with LPS, Polyinosinic-Polycytidylic Acid and Pam3CSK

  AUTHORS: Thongchai Taechowisan, Srisakul Chanaphat, Wanwikar Ruensamran, Waya S. Phutdhawong

  KEYWORDS: 3-Methylcarbazoles; Anti-Inflammatory Activity; RAW 264.7 Cells, Streptomyces sp.

  JOURNAL NAME: Advances in Microbiology, Vol.2 No.2, June 13, 2012

  ABSTRACT: In the present study, 3-methylcarbazole and 1-methoxy-3-methylcarbazole were isolated from the culture of Streptomyces sp. LJK109, endophyte of Alpinia galanga Swartz. 3-methylcarbazole, a carbazole derivative, has been found to be highly potent as anti-inflammatory agent. The immunomodulatory activity of these agents in toll like receptor (TLR)-activated RAW 264.7 macrophages induced by lipopolysaccharide (LPS), Poly(I:C), and pam3CSK was investigated by assessing nitric oxide (NO) and pro-inflammatory cytokines. The 3-methylcarbazoles dose-dependently suppressed the release of NO, PGE2, TNF-α, IL-1β, IL-6 and IL-10 in LPS- and pam3CSK-activated macrophages but not in Poly(I:C)-activated macrophages. Our results suggest that 3-methylcarbazoles can be further developed as a promising anti-inflammatory remedy.