TITLE:
Validation of a HPLC Method for Determination of Glutamine in Food Additives Using Post-Column Derivatization
AUTHORS:
Marlus Chorilli, Hérida Regina Nunes Salgado, Fabiana De Santana Santos, Lucélia Magalhães Da Silva
KEYWORDS:
Glutamine; Food Additive Product; Liquid Chromatography
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.3 No.2,
February
22,
2012
ABSTRACT: An ion-exchange chromatography (IEC) followed by post-column derivatization method was validated for the determination of glutamine in food additive products. The chromatographic method was carried out on a sodium cation-exchange column and using detection at 570 nm. The mobile phase consisted of sodium eluant, pH 3.15, with 5% sulfolane, sodium eluant, pH 7.40 and sodium column regenerant. The method was linear in the range of 4 - 50 μg/mL (r2 = 0.9999). The accuracy was 99.78%. Moreover, method validation demonstrated acceptable results for precision and robustness. The method was found to be able to use for routine analysis of the glutamine.