Validation of a HPLC Method for Determination of Glutamine in Food Additives Using Post-Column Derivatization

DOI: 10.4236/ajac.2012.32016   PDF   HTML     8,293 Downloads   16,555 Views   Citations

Abstract

An ion-exchange chromatography (IEC) followed by post-column derivatization method was validated for the determination of glutamine in food additive products. The chromatographic method was carried out on a sodium cation-exchange column and using detection at 570 nm. The mobile phase consisted of sodium eluant, pH 3.15, with 5% sulfolane, sodium eluant, pH 7.40 and sodium column regenerant. The method was linear in the range of 4 - 50 μg/mL (r2 = 0.9999). The accuracy was 99.78%. Moreover, method validation demonstrated acceptable results for precision and robustness. The method was found to be able to use for routine analysis of the glutamine.

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M. Chorilli, H. Salgado, F. Santos and L. Silva, "Validation of a HPLC Method for Determination of Glutamine in Food Additives Using Post-Column Derivatization," American Journal of Analytical Chemistry, Vol. 3 No. 2, 2012, pp. 113-117. doi: 10.4236/ajac.2012.32016.

Conflicts of Interest

The authors declare no conflicts of interest.

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