World Health Organization (2016) Global Report on Diabetes.
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TITLE:
Tephrosia purpurea Fraction Attenuates Lipid Accumulation and Adipogenesis in 3T3-L1 Adipocytes and Reduces Body Weight in High Fat Diet Induced Obese Rats
AUTHORS:
Karunakaran Reddy Sankaran, Lokanatha Oruganti, Krishna Chintakuntla, Balaji Meriga
KEYWORDS:
Body Weight, Cell Viability, Enzyme Inhibition, Lipolysis, 3T3-L1 Cells
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.10 No.9,
September
30,
2019
ABSTRACT: The anti-adipogenic and anti-obesity activity of
chloroform fraction of Tephrosia purpurea (CFTp) on 3T3-L1 adipocytes and high fat diet
(HFD)-fed obese rats was evaluated in this study. A substantial and dose
dependent inhibition of α-glucosidase
(81%) and lipase (75%) activities by CFTp was noticed. Treatment with CFTp (250
μg/mL) significantly inhibited 3T3-L1 adipocytes differentiation and lipid
accumulation. A semi-quantitative
RT-PCR analysis of 3T3-L1 cells revealed down regulation of mRNA expression of
peroxisome proliferator-activated receptor-γ (PPAR-γ), fatty acid synthase (FAS)
and acetyl CoA carboxylase-2 (ACC-2), while
glucose transporter type-4 (GLUT-4) expression was up-regulated in a
dose dependent manner with CFTp. Further, oral administration of CFTp (200
mg/kg.b.wt.) significantly reduced body weight gain, fat mass, blood glucose and
leptin levels in high fat diet (HFD)-induced obese rats. Taken together, these
findings demonstrate that CFTp possesses potent anti-obesity activities.
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