TITLE:
Effect of the Aqueous Extract of Schumanniophyton magnificum Harms on Sexual Maturation and Fertility of Immature (K. schum) Female Rat
AUTHORS:
Emma F. Bend, Pierre C. Oundoum Oundoum, Marie I. Ngaha Njila, Brice L. Koloko, Camille D. Nyonseu, Samuel H. Mandengue, Paul Moundipa, Theophile Dimo, Dieudonne Massoma Lembè
KEYWORDS:
Schumanniophyton magnificum, Sexual Maturation, Hormone Level, Fertility
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.9 No.10,
October
24,
2018
ABSTRACT:
The aim of the study was to bring scientific
evidence of the curative action of the stem bark aqueous extract of Schumanniophyton
magnificum (ASMa) on the sexual maturation and fertility of the immature
female Wistar rat. Forty immature female rats were randomized and divided into
4 groups of ten animals each and orally treated with the ASMa at doses of 0
(distilled water), 200 mg/kg, 400 mg/kg and 800 mg/kg /BW/day for 30 consecutive days. Body weight and food intake were
recorded throughout the experimental period. The precocity of the puberty onset
in treated animals was evaluated through the determination of their age at
vaginal opening. At the end of the experimental period, 5 animals in each group
were sacrificed and blood samples were collected for hormonal assay. Their
ovaries and uteri were removed, blotted, weighted and prepared for biochemical analysis.
The remaining rats (5 per group) were crossed with males of proven fertility.
After laparotomy (ten days after mating) and delivery, fertility and
gestational parameters were recorded. It was noticed that, body weight gain
increased significantly at all doses although there was no significant difference in food intake. The sexual maturation of
treated animal was reduced to 5 days when compared to control. This was
associated with the simultaneous elevation of FSH and LH (p mg/kg. However, the ovarian cholesterol significantly decreased (p mg/kg and 800 mg/kg. The animal treated at
doses of 400 mg/kg and 800 mg/kg exhibited an early fertilization (2 - 3 days) when compared to the control one (9 - 14 days). The number of implantation site significantly increased (p mg/kg and 800 mg/kg) after laparotomy as
well as the number of alive fetuses after delivery and gestational rate (80%
and 100%) respectively. These results provide the strong evidence on the
induction of the onset puberty and gonadotropin synthesis, the improvement of
the ovarian folliculogenesis and the fertility effect of ASMa in immature rats.