TITLE:
Ameliorative Potential of Mesenchymal Stem Cells and Hematopoietic Stem Cells against CCl4 Induced Chromosomal Abnormalities in Experimental Rat Liver Fibrosis
AUTHORS:
Rokaya H. Ahmed, Nagwa K. Roshdy, Hayam E. Saleh, Mohammed I. Aref, Nermeen M. Hassan, Noura R. Mohammed
KEYWORDS:
Cytogenetic and Stem Cells, CCl4 and Chromosomal Aberrations
JOURNAL NAME:
Stem Cell Discovery,
Vol.4 No.4,
September
16,
2014
ABSTRACT: Background and Objective:
The liver is responsible for metabolism and detoxification of the most of
components that enter the body. Once the liver became injured, its efficient
treatment with famous chemical drugs was limited. Therefore, interest concerned
the use of alternative medicines for the treatment of hepatic disease has been
arisen. The present study was aimed to investigate the therapeutic effect of
the two types of stem cells against chromosomal aberrations in bone marrow (BM)
cells of rats treated with carbon tetrachloride (CCl4). Design and
Method: BM-derived mesenchymal stem cells (MSCs) were isolated and propagated
in culture for 2 weeks and were characterized morphologically. Human umbilical
cord blood (UCB) cells were obtained after full-term caesarean delivery from
healthy donors. Low-density mononuclear cells were separated over Ficoll-Paque,
and CD34+ hematopoietic
cells were isolated using a magnetic cell sorter. Rats were divided into 4
groups: control, CCl4, CCl4 plus
MSC, and CCl4 plus
CD34+. Liver tissue was examined histopathologically for all groups.
Results: The results of the present study indicated a significant increase (p 0.05) in the number of metaphases with different types of chromosomal
aberrations in CCl4 group.
Treatment of the animals with BM-MSCs and UCB-CD34+ cells improved both genotoxicity and
histopathological changes induced by CCl4.