Aida, J., Yokoyama, A., Shimomura, N., Nakamura, K., Ishikawa, N., et al. (2013) Telomere Shortening in the Esophagus of Japanese Alcoholics: Relationships with Chromoendoscopic Findings, ALDH2 and ADH1B Genotypes and Smoking History. Public Library of Science One, 8, e63860.

has been cited by the following article:

• TITLE: Determination of Telomere Length by the Quantitative Fluorescence in Situ Hybridization (Q-FISH) Method

  AUTHORS: Junko Aida, Naotaka Izumiyama-Shimomura, Ken-ichi Nakamura, Naoshi Ishikawa, Masanori Terai, Yoko Matsuda, Shinsuke Aida, Tomio Arai, Kaiyo Takubo

  KEYWORDS: Telomere, Telomere Length, Q-FISH, Tissue Section, Chromosome

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.5 No.12, August 26, 2014

  ABSTRACT: Telomeres are nucleoprotein complexes located at the ends of eukaryotic chromosomes and are shorten with aging or various causes. Shortened telomere plays an important role for chromosomal instability in carcinogenesis, or a number of diseases in relation to aging. FISH using peptide-nucleic acid (PNA) probe is suitable for analyzing telomeres, because telomere is a part of DNA molecule and localized in chromosomal end in loop shape structure (T-loop). We introduce our telomere analysis for tissue sections and cultured cells in this paper. On the tissue sections, we analyze telomere length with telomere intensity to centromere intensity ratio (TCR), centromere as an inner control. In the cultured cells, we analyze telomere lengths on each chromosome with karyotyping. Those Q-FISH methods by PNA probe are an accurate and reliable tool for research on telomere lengths in various conditions in biology.