TITLE:
Impact of Different Carbohydrates and Their Concentrations on in Vitro Regeneration of Solanum viarum (Dunal)—An Important Anticancer Medicinal Plant
AUTHORS:
Manikyam Doraiswamy Naidu Mahadev, Chandra Sekhar Panathula, ChallagundlaVaradarajulu Naidu
KEYWORDS:
Solanum viarum; Carbohydrates; Plant Regeneration; Multiple Shoot Induction
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.1,
January
22,
2014
ABSTRACT:
In this present study, the effect of various carbon sources such as
sucrose, glucose, fructose and maltose was investigated on in vitro shoot regeneration of Solanum viarum using axillary bud explants. The
frequency, growth and multiplication rate were highly influenced by the type
and concentration of carbon source used. Among the different concentrations (1%-6%) of
carbohydrates studied, the maximum number of shoots (22.6 ± 0.50) and
shoot length (5.92 ± 0.13 cm) was obtained on MS medium supplemented
with 4% (w/v) fructose. The least number of shoots (1.5 ± 0.32) was
obtained on 6% maltose and the least shoot length (1.2 ± 0.23) was
observed on 6% glucose. Among the four types of carbon sources that were employed in
the present study, fructose at 4% proved to be better choice for multiple shoot
regeneration followed by sucrose, glucose and maltose from axillary bud
explants of S. viarum.