In vitro propagation of Aegle marmelos (L.) corr., a medicinal plant through axillary bud multiplication


A protocol for rapid in vitro propagation from nodal explants of the medicinal tree species Aegle marmelos (L.) corr. of family Rutaceae has been described. High frequency bud break were induced on Murashige and Skoog’s (1962) basal medium supplemented with 0.5 mg benzyladenine (BA)/l. After 10 days of culture, nodal explants with multiplied buds started callusing with restricted growth and defoliation. When the same nodal explants ware transferred into the same basal medium supplemented with 0.5 mg BA/l with different concentrations of either kinetin (KN) or gibberellic acid (GA3) or in combinations has shown healthy shoots with expanded shoot length. Excised shoots (2cm-3cm long with 2 to 3 nodes) when grown on 1/2 MS basal medium with 2.5 mg/l indole-3-butyric acid (IBA) and 0.5% activated charcoal (A.C.)/l has shown rhizogenesis. After excision, in the second passage, the nodal explants also showed bud break when sub cultured on MS basal medium supplemented with 0.5 mg BA/l. These shoots also successfully rooted on the same above said medium.

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Puhan, P. and Rath, S. (2012) In vitro propagation of Aegle marmelos (L.) corr., a medicinal plant through axillary bud multiplication. Advances in Bioscience and Biotechnology, 3, 121-125. doi: 10.4236/abb.2012.32018.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Karawya, M.S., Mirchom, Y.W. and Shehuata, I.A. (1980) Sterols, triterpenes, Coumarins and alkaloids of Aegle marmelos correa, cultivated in Egypt. Egyptian Journal of Pharmaceutical Sciences, 21, 239-248.
[2] Tokitomo, Y., Shimono, Y., Kobayashi, A. and Yamanishi, T. (1982) Aroma components of bael fruit (Aegle marmelos correa). Agricultural Biology and Chemistry, 46, 1873-1877. doi:10.1271/bbb1961.46.1873
[3] Pattnaik, S., Subramanyam, V.R. and Kale, C. (1996) Antibacterial and antifungal activity of ten essential oils in vitro. Microbios, 86, 237-246.
[4] Parichha, S. (2004) Bael (Aegle marmelos) nature’s most natural medicinal fruit. Orissa Review, 9, 16-17.
[5] Bera, T.K. and Roy, S.C. (1993) Micropropagation of Tylophora Indica (Burmn f.) Merr. by multiple bud formation from mature leaf explants without callus intervention. Botanical Bulletin of Academia Sinica, 34, 83-87.
[6] Sudha, G.C. and Seeni, S. (1994) In vitro multiplication and field establishment of Adhatoda beddomei CB clarke, a rare medicinal plants. Plant Cell Reports, 13, 203-207. doi:10.1007/BF00239893
[7] Sahoo, Y. and Chand, P.K. (1998) Micropropagation of Vitex negundo L., a woody aromatic medicinal shrub, through high frequency axillary shoot proliferation. Plant Cell Reports, 18, 301-307. doi:10.1007/s002990050576
[8] Wawrosch, C., Maskay, N. and Kopp, B. (1998) Micropropagation of the threatened nepalese medicinal plant Swertia chirwta Buch.-Hamex. wall. Plant Cell Reports, 18, 997-1001. doi:10.1007/s002990050697
[9] Ahuja, M.R. (1991) Biotechnology of forest trees. Plant Research and Development, 33, 106-120.
[10] Shankar, S. and Mohan Ram, H.Y. (1990) In vitro Plantlet regeneration in the leguminous tree Sesbania sesban. Phytomorphology, 40, 43-52.
[11] Malathy, S. and Pai, J.S. (1998) Micropropagation of Ixora Singaporensiss (Linn.)—An ornamental shrub. Current Science, 75, 545-547.
[12] Patnaik, K. and Debata, B.K. (1996) Micropropagation of Hemidesmus Indicus (L.) R. BR: Through axillary bud culture. Plant Cell Reports, 15, 427-430. doi:10.1007/BF00232069
[13] Quraishi, A., Koche, V. and Mishra, S.K. (1996) In vitro micropropagation from nodal segments of Cleistanthus collinus. Plant Cell, Tissue and Organ Culture, 45, 87-91. doi:10.1007/BF00043433
[14] Thirunavoukkarasu, M. and Debata, B.K. (1998) Micro propagation of Glemelina arborea Roxb through axillary bud culture. Indian Journal of Plant Physiology, 3, 82-85.
[15] Selvakumar, V., Anbudurai, R.P. and Balkumar, T. (2001) In vitro propagation of the medicinal plant Plumbago zeylanica L. through nodal explants. In Vitro Cellular & Developmental Biology—Plant, 37, 280-284. doi:10.1007/s11627-001-0050-x
[16] Raha, S. and Roy, S.C. (2001) In vitro plant regeneration in Holarrhena antidysenterica wall, through high frequency axillary shoot proliferation. In Vitro Cellular & Developmental Biology—Plant, 37, 232-236. doi:10.1007/s11627-001-0041-y
[17] Bhati, R., Sekhwatt, N.S. and Arya, H.C. (1992) In vitro regeneration of Plant lets from root segments of Aegle marmelos. Indian Journal of Experimental Biology, 30, 844-845.
[18] Hossain, M., Islam, R., Islam, A. and Joarder, O.I. (1995) Direct organogenesis in cultured hypocotyl explants of Aegle marmelos corr. Plant Tissue Culture, 5, 21-25.
[19] Arumugam, A. and Rao, M.V. (1996) In vitro production of plantlets from cotyledonary node cultures of Aegle marmelos L. Corr. Advances in Plant Sciences, 9, 181- 186.
[20] Islam, R., Hossain, M., Joarder, O.I. and Karim, M.R. (1993) Adventitious shoot formation on excised leaf explants of in vitro grown seedlings of Aegle marmelos. Corr. Journal of Horticultural Science, 68, 495-498.
[21] Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiology, 15, 473-497. doi:10.1111/j.1399-3054.1962.tb08052.x
[22] Duncan, D.B. (1955) Multiple range and multiple F test. Biometrics, 11, 1-42. doi:10.2307/3001478
[23] Purohit, S.D., Dave, A. and Kukda, G. (1995) Micropropagation of safed musli (Chlorophytum borivilianum), a rare medicinal herb. Plant Cell, Tissue and Organ Culture, 39, 93-96. doi:10.1007/BF00037596
[24] Sen, J. and Sharma, A.K. (1991) Micropropagation of Withania somnifera from germinating seeds and shoot tips. Plant Cell, Tissue and Organ Culture, 26, 71-73. doi:10.1007/BF00036108
[25] Das, D.K., Shiva Prakash, N. and Bhalla-Sarin, N. (1999) Multiple shoot induction and plant regeneration in litchi (Litchi chinensis Sonn.). Plant Cell Reports, 18, 691-695. doi:10.1007/s002990050644
[26] Bogetti, B., Jasik, J. and Mantell, S. (1999) In vitro multiplication of cashew (Anacardium occidentale L.) using shoot node explants of glasshouse-raised plants. Plant Cell Reports, 18, 456-461. doi:10.1007/s002990050603
[27] Khosh-Khui, M. and Sink, K.C. (1982) Callus induction and culture of Rosa. Scientia Horticulturae, 17, 361-370. doi:10.1016/0304-4238(82)90117-0
[28] Khosh-Khui, M. and Sink, K.C. (1982) Rooting enhancement of Rosa hybrida for tissue culture propagation. Scientia Horticulturae, 17, 371-376. doi:10.1016/0304-4238(82)90118-2
[29] Rout, G.R., Debata, B.K. and Das, P. (1990) In vitro Clonal multiplication of roses. Proceedings of the National Academy of Sciences (India), 60, 311-318.
[30] Ajithkumar, D. and Seeni, S. (1998) Rapid clonal multiplication through in vitro axillary shoot proliferation of Aegle marmelos (L.) Corr., a medicinal tree. Plant Cell Reports, 17, 422-426.
[31] Len, L.C. and Kong, L.S. (1985) Micropropagation of Lagestroemia speciosa (L.) Pers (lythraccae). Gardens’ Bull, 38, 175-184. doi:10.1007/s002990050418

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