•   Home
  • Proceedings
  • International Workshop on Diffuse Pollution-Management Measu...

International Workshop on Diffuse Pollution-Management Measures and Control Technique (IWDCT 2010 E-BOOK)

Huainan,China,10.27-10.29,2010

ISBN: 978-1-935068-38-9 Scientific Research Publishing, USA

E-Book 394pp Pub. Date: October 2010

Category: Earth & Environmental Sciences

Price: $70

Title: Dectection of Pesticides Using Microcantilever Immunosensor
Source: International Workshop on Diffuse Pollution-Management Measures and Control Technique (IWDCT 2010 E-BOOK) (pp 111-115)
Author(s): Changguo Xue, Key Laboratory of Mechanical Behavior and Design of Material of Chinese Academy of Sciences University of Science and Technology of China Hefei, People’s Republic of China
Qingchuan Zhang, Key Laboratory of Mechanical Behavior and Design of Material of Chinese Academy of Sciences University of Science and Technology of China Hefei, People’s Republic of China
Hongwei Zhao, College of Agronomy and Biotechnology China Agricultural University Beijing, People’s Republic of China
Baomin Wang, College of Agronomy and Biotechnology China Agricultural University Beijing, People’s Republic of China
Abstract: A microcantilever immunosensor which has been the development for label-free detection of pesticides was exemplified with the ethyl parathion (EP). Sulfhydrylated anti-EP antibody was immobilized on the gold surface of the microcantilever for the measurement of EP. Subsequently, the deflection of the microcantilever corresponding to different EP concentrations was real time monitored by optical lever technique. The activity of sulfhydrylated antibody immobilized on the gold surface of a microcantilever was evaluated by non-competitive enzyme-linked immunosorbent assay (ELISA).The experimental results indicated: although the sulfhydrylation caused loss of 19.9% activities of mAbs, sulfhydrated anti-EP antibody on the gold surface can keep the activity. Deflection of microcantilever is linear to EP concentration from 10~500 ng/mL. The limit of detection is 1.7 ng/mL.
Proceedings by Subjects
Resources
Products
Contact us
book@scirp.org
WhatsApp +86 18163351462(WhatsApp)
Click here to send a message to me 1243940697
Book Publishing WeChat
Follow SCIRP
Free SCIRP Newsletters
Copyright © 2006-2024 Scientific Research Publishing Inc. All Rights Reserved.
Top