Author(s): |
Guangliang Tian, Institute of Solar Energy; Engineering R-search Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education; Provincial Key Laboratory of bioenergy and Environmental Biotechnology; Provincial Key Laboratory of Rural Energy En Shiqing Liu, Institute of Solar Energy; Engineering R-search Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education; Provincial Key Laboratory of bioenergy and Environmental Biotechnology; Provincial Key Laboratory of Rural Energy En Fang Yin, Institute of Solar Energy; Engineering R-search Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education; Provincial Key Laboratory of bioenergy and Environmental Biotechnology; Provincial Key Laboratory of Rural Energy En Rui Xu, Institute of Solar Energy; Engineering R-search Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education; Provincial Key Laboratory of bioenergy and Environmental Biotechnology; Provincial Key Laboratory of Rural Energy En Yumin Xu, Institute of Solar Energy; Engineering R-search Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education; Provincial Key Laboratory of bioenergy and Environmental Biotechnology; Provincial Key Laboratory of Rural Energy En Yubao Chen, Institute of Solar Energy; Engineering R-search Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education; Provincial Key Laboratory of bioenergy and Environmental Biotechnology; Provincial Key Laboratory of Rural Energy En Wudi Zhang, Institute of Solar Energy; Engineering R-search Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education; Provincial Key Laboratory of bioenergy and Environmental Biotechnology; Provincial Key Laboratory of Rural Energy En |
Abstract: |
through three-factor (high gravity ethanol, high gravity sodium chloride and heat) joint shock test, we found that different yeast species have different sensitivity to temperature. For example, the mutants resulting from 45℃ and 50℃shock of rice wine yeast can ferment in medium containing 17% ethanol, and they have similar ethanol production ability; but the mutant resulting from 50℃ shock of brewer’s yeast can produce more ethanol than mutant resulting from 45℃ shock. Through 48 hours fermentation, original rice yeast can produce 2.6% alcohol in mash, while some of the mutants from this yeast can produce 3.1% alcohol; original brewer's yeast can produce 2.9% alcohol in mash, while some of the mutants of brewer's yeast can produce 3.8% alcohol. So the way of three-factor joint shock can improve yeast ability to produce ethanol and ethanol tolerant capacity significantly.
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