Antioxidant and Antimicrobial Activities of Essential Oils Extracted from Laurus nobilis L. Leaves by Using Solvent-Free Microwave and Hydrodistillation


In this study, laurel essential oils were obtained by using solvent-free microwave extraction (SFME) and hydrodistillation (HD) methods from Laurus nobilis leaves and determined their antioxidant and antimicrobial activity. Extraction time was reduced by about 43% in SFME at 622 W and 67% in SFME at 249 W compared to hydrodistillation. Essential oil of laurel was extracted by SFME at 622 W (100%) and 249 W (40%) power levels and HD inhibited oxidation generated by ABTS radical by 93.88%, 94.13% and 92.06%, respectively. Trolox equivalent antioxidant capacities (TEAC) of essential oils were 0.18 mM/mL oil for SFME at 622 W, 1.36 mM/mL oil for SFME at 249 W and 2.40 mM/mL oil for HD (p < 0.05). Essential oils of L. nobilis were extracted by SFME at 100% and 40% power levels and HD inhibited linoleic acid peroxidation by 70.57%, 63.53% and 89.18% respectively. Inhibition effects of laurel essential oils obtained by SFME at different power levels and HD on DPPH radical cation oxidation were not significantly different. The strongest antioxidant activity against DPPH radical was found in the essential oil obtained by SFME at 100% power level. Essential oils displayed antimicrobial activity against Staphylococcus aureus 6538P, Escherichia coli O157:H7 and Salmonella typhimurium NRRL E 4463 except for Listeria monocytogenes. The inhibitory effect on Staphylococcus aureus 6538P survival of laurel oil obtained from SFME by using lower power level was found to be lower than that obtained from SFME at 100% power level and HD (p < 0.05).

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S. El, N. Karagozlu, S. Karakaya and S. Sahın, "Antioxidant and Antimicrobial Activities of Essential Oils Extracted from Laurus nobilis L. Leaves by Using Solvent-Free Microwave and Hydrodistillation," Food and Nutrition Sciences, Vol. 5 No. 2, 2014, pp. 97-106. doi: 10.4236/fns.2014.52013.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] L. Langseth, “OxIdants, AntIoxIdants, and DIsease PreventIon,” ILSI Europe Press, 2000.
[2] H. W. Kang, K. W. Yu, W. J. Jun, I. S. Cahng, S. B. Han, H. Y. KIm and H. Y. Cho, “IsolatIon and CharacterIzatIon of Alkyl Peroxy RadIcal ScavengIng Compound from Leaves of Laurus nobIlIs,” ChemIcal and PharmaceutIcal BulletIn, Vol. 25, No. 1, 2002, pp. 102-108.
[3] S. Santoyo, R. Lloría, L. JaIme, E. Ibanez, F. J. Senoráns and G. Reglero, “SupercrItIcal FluId ExtractIon of AntIoxIdant and AntImIcrobIal Compounds from Laurus nobIlIs L. ChemIcal and FunctIonal CharacterIzatIon,” European Food Research and Technology, Vol. 222, No. 5, 2006, pp. 565-571.
[4] M. Elmastas, I. GülcIn, O. Isildak, O. I. KüfrevIoglu, K. Ibaoglu and H. Y. Aboul-EneIn, “AntIoxIdant CapacIty of Bay (Laurus nobIlIs L.) Leave Extracts,” Journal of the IranIan ChemIcal SocIety, Vol. 3, No. 3, 2006, pp. 258266.
[5] P. PIllaI and K. Ramaswamy, “Effect of Naturally OccurIng AntImIcrobIals and ChemIcal PreservatIves on the Growth of AspercIllus parasItIcus,” Journal of Food ScIence and Technology, 2011.
[6] M. Skerget, P. KotnIk, M. HadolIn, A. R. HraS, M. SImonIc and Z. Knez, “Phenols ProanthocyanIdIns Flavones and FlavonoIds In Some Plant MaterIals and TheIr AntIoxIdant ActIvItIes,” Food ChemIstry, Vol. 89, No. 2, 2005, pp. 191-198.
[7] H. Yalcin, M. Akin, M. Sanda and A. Cakir, “Gas Chromatography/Mass Spectrometry AnalysIs of Laurus nobIlIs EssentIal OIl ComposItIon of Northern Cyprus,” Journal of MedIcInal Food, Vol. 10, No. 4, 2007, pp. 715-719.
[8] M. Polovka and M. Suhaj, “DetectIon of Caraway and Bay Leaves IrradIatIon Based on TheIr Extracts’ AntIoxIdant PropertIes EvaluatIon,” Food ChemIstry, Vol. 119, No. 1, 2010, pp. 391-401.
[9] M. KarpInska-Tymoszezyk, “The Effects of OIl Soluble Rosemary Extract, SodIum Erythorbate and a MIxture of OIl-Soluble Rosemary Extract and SodIum Erythorbate on the QualIty of Cooked Meatballs,” Journal of Food ScIence and Technology, Vol. 50, No. 3, 2013, pp. 443454.
[10] F. ConfortI, G. StattI, D. Uzunov and F. MenIchInI, “ComparatIve ChemIcal ComposItIon and AntIoxIdant ActIvItIes of WIld and CultIvated Laurus nobIlIs L. Leaves and FoenIculum vulgare subsp. pIperItum (UcrIa) CoutInho Seeds,” BIologIcal & PharmaceutIcal BulletIn, Vol. 29, 2006, pp. 2056-2064.
[11] S. A. Burt and R. D. ReInders, “AntIbacterIal ActIvIty of Selected Plant EssentIal OIls agaInst EscherIchIa colI O157:H7,” Letters In ApplIed MIcrobIology, Vol. 36, No. 3, 2003, pp. 162-167.
[12] M. SImIc, N. KundakovIc and N. KovacevIc, “PrelImInary Assay on the AntIoxIdatIve ActIvIty of Laurus nobIlIs Extracts,” FItoterapIa, Vol. 74, No. 6, 2003, pp. 613616.
[13] A. FerreIra, C. Proenc, M. L. M. SerralheIro and M. E. M. Araújo, “The In VItro ScreenIng for AcetylcholInesterase InhIbItIon and AntIoxIdant ActIvIty of MedIcInal Plants from Portugal,” Journal of Ethnopharmacology, Vol. 108, No. 1, 2006, pp. 31-37.
[14] M. A. Ferhat, N. TIgrIne-KordjanI, S. Chemat, B. Y. MeklatI and F. Chemat, “RapId ExtractIon of VolatIle Compounds UsIng a New SImultaneous MIcrowave DIstIllatIon: Solvent ExtractIon DevIce,” ChromatographIa, Vol. 65, No. 3-4, 2007, pp. 217-222.
[15] B. Bayramoglu, S. SahIn and G. Sumnu, “ExtractIon of EssentIal OIl from Laurel Leaves by UsIng MIcrowaves,” SeparatIon ScIence and Technology, Vol. 44, No. 3, 2009, pp. 722-733.
[16] C. Buffler, “MIcrowave CookIng and ProcessIng: EngIneerIng Fundamentals for the Food ScIentIsts,” AVI Book Press, New York, 1993.
[17] R. Re, N. PelegrInI, A. Proteggente, A. Pannala, M. Yang and C. RIce-Evans, “AntIoxIdant ActIvIty ApplyIng an Improved ABTS RadIcal CatIon DecolorIzatIon Assay,” Free RadIcal BIology and MedIcIne, Vol. 26, No. 9-10, 1999, pp. 1231-1237.
[18] N. J. MIller, C. A. RIce-Evans, M. J. DavIes, V. GopInathan and A. MIlner, “A Novel Method for MeasurIng AntIoxIdant CapacIty and Its ApplIcatIon to MonItorIng the AntIoxIdant Status In Premature Neonates,” ClInIcal ScIence, Vol. 84, No. 4, 1993, pp. 407-412.
[19] A. Braca, N. D. TommasI, L. D. BarI, C. PIzza, M. PolItI and I. MorellI, “AntIoxIdant PrIncIples from BauhInIa TerapotensIs,” Journal of Natural Products, Vol. 64, No. 7, 2001, pp. 892-895.
[20] J. M. Kuo, D. B. Yeh and B. S. Pan, “RapId PhotometrIc Assay EvaluatIng AntIoxIdatIve ActIvIty In EdIble Plant MaterIal,” Journal of AgrIcultural and Food ChemIstry, Vol. 47, No. 8, 1999, pp. 3206-3209.
[21] M. Güllüce, F. SahIn, M. Sokmen, H. Ozer, D. Daferera, A. Sokmen, M. PolIssIou, A. AdIguzel and H. Ozkan, “AntImIcrobIal and AntIoxIdant PropertIes of the EssentIal OIls and Methanol Extract from Mentha longIfolIa L. ssp LongIfolIa,” Food ChemIstry, Vol. 103, No. 4, 2007, pp. 1449-1456.
[22] M. R. Hubbard, “StatIstIcal QualIty Control for the Food Industry,” Van Nostrand ReInhold Press, New York, 1990.
[23] O. Talaz, I. GülcIn, S. GOksu and N. Saracoglu, “AntIoxIdant ActIvIty of 5,10-DIhydroIndeno[1,2-b]Indoles ContaInIng SubstItuents on DIhydroIndeno Part,” BIoorganIc & MedIcInal ChemIstry, Vol. 17, No. 18, 2009, pp. 65836589.
[24] S. Karakaya, S. N. El, N. Karagozlu and S. SahIn, “AntIoxIdant and AntImIcrobIal ActIvItIes of EssentIal OIls ObtaIned from Oregano (OrIganum vulgare ssp. hIrtum) by UsIng DIfferent ExtractIon Methods,” Journal of MedIcInal Food, Vol. 14, No. 6, 2011, pp. 645-652.
[25] A. ünver, D. Arslan, M. M. Ozcan and M. Akbulut, “PhenolIc Content and AntIoxIdant ActIvIty of Some SpIces,” World ApplIed ScIences Journal, Vol. 6, No. 3, 2009, pp. 373-377.
[26] B. Shan, Y. CaI, J. D. Brooks and H. Corke, “The In VItro AntIbacterIal ActIvIty of DIetary SpIce and MedIcInal Herb Extracts,” InternatIonal Journal of Food MIcrobIology, Vol. 117, No. 1, 2007, pp. 112-119.
[27] W. Brand-WIllIams, M. E. CuvelIer and C. Berset, “Use of a Free RadIcal Method to Evaluate AntIoxIdant ActIvIty,” LebensmIttel-WIssenschaft & TechnologIe, Vol. 28, No. 1, 1995, pp. 25-30.
[28] I. HInneburg, H. J. DamIen Dorman and R. HIltunen, “AntIoxIdant ActIvItIes of Extracts from Selected CulInary Herbs and SpIces,” Food ChemIstry, Vol. 97, No. 1, 2006, pp. 122-129.
[29] T. A. MIsharIna and A. N. Polshkov, “AntIoxIdant PropertIes of EssentIal OIls: AutoxIdatIon of EssentIal OIls from Laurel and Fennel and of TheIr MIxtures wIth EssentIal OIl from CorIander,” ApplIed BIochemIstry and MIcrobIology, Vol. 41, No. 6, 2005, pp. 610-618.
[30] O. PolIteo, M. JukI and M. MIlo, “ChemIcal ComposItIon and AntIoxIdant ActIvIty of Free VolatIle Aglycones from Laurel (Laurus nobIlIs L.) Compared to Its EssentIal OIl,” CroatIca ChemIca Acta, Vol. 80, No. 1, 2007, pp. 121126.
[31] S. SahIn, G. Sumnu, E. Yener, S. Karakaya, S. N. El and N. Karagozlu, “ExtractIon of EssentIal OIls from SpIces UsIng Novel TechnologIes and PhysIcal AntIoxIdant and AntImIcrobIal PropertIes of These OIls,” TUBITAK (The ScIentI?c and TechnologIcal Research CouncIl of Turkey), Ankara, 2009, Project Number 104 O 265.
[32] A. SImIc, D. SokovIc, M. RIstIc, S. GrujIc-JovanovIc, J. VukojevIc and P. D. MarIn, “The ChemIcal ComposItIon of Some Lauraceae EssentIal OIls and TheIr AntIfungal ActIvItIes,” Phytotherapy Research, Vol. 18, No. 9, 2004, pp. 713-717.
[33] H. DerwIch, Z. BenzIane and A. BoukIr, “ChemIcal ComposItIon and AntIbacterIal ActIvIty of Leaves EssentIal OIl of Laurus nobIlIs from Morocco,” AustralIan Journal of BasIc and ApplIed ScIences, Vol. 3, No. 4, 2009, pp. 3818-3824.
[34] O. O. Okoh, A. P. SadImenko and A. J. Afolayan, “ComparatIve EvaluatIon of the AntIbacterIal ActIvItIes of the EssentIal OIls of RosmarInus offIcInalIs L. ObtaIned by HydrodIstIllatIon and Solvent Free MIcrowave ExtractIon Methods,” Food ChemIstry, Vol. 120, No. 1, 2010, pp. 308-312.
[35] M. Mu?oz, L. Guevaral, A. Palop, J. Tabera and P. S. Fernández, “DetermInatIon of the Effect of Plant EssentIal OIls ObtaIned by SupercrItIcal FluId ExtractIon on the Growth and VIabIlIty of LIsterIa Monocytogenes In Broth and Food System UsIng Flow Cytometry,” LWT—Food ScIence and Technology, Vol. 42, No. 1, 2009, pp. 220227.
[36] O. SagdIc, A. Kuscu, M. Ozcan and S. OzcelIk, “Effects of TurkIsh SpIce Extracts at VarIous ConcentratIons on the Growth of EsherIchIa colI 0157:H7,” Food MIcrobIology, Vol. 19, No. 5, 2002, pp. 473-480.
[37] O. Sagdic, “SensItIvIty of Four PathogenIc BacterIa to TurkIsh Thyme and Oregano Hydrosols,” LebensmIttelWIssenschaft & TechnologIe, Vol. 36, No. 5, 2003, pp. 467-473.
[38] I. DadalIoglu and G. A. EvrendIlek, “ChemIcal ComposItIons and AntIbacterIal Effects of EssentIal OIls of TurkIsh Oregano, Bay Laurel, SpanIsh Lavender, and Fennel and Common Foodborne Pathogens,” Journal of AgrIcultural and Food ChemIstry, Vol. 52, No. 26, 2004, pp. 8255-8260.
[39] M. Ozcan and O. Erkmen, “AntImIcrobIal ActIvIty of the EssentIal OIls of TurkIsh Plant SpIces,” European Food Research and Technology, Vol. 212, No. 6, 2001, pp. 658660.
[40] O. Sagdic and M. Ozcan, “AntIbacterIal ActIvIty of TurkIsh SpIce Hydrosols,” Food Control, Vol. 14, No. 3, 2003, pp. 141-143.

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