Identification of a SSR marker (TOM-144) linked to Fusarium wilt resistance in Solanum lycopersicum

Pritesh Parmar, Ankit Sudhir, R. Preethi, Bhaumik Dave, Ketankumar Panchal, Ramalingam B. Subramanian, Arvind Patel, K. B. Kathiria

B. R. D. School of Biosciences, Sardar Patel University, Vallabh Vidyanagar, India.
Vegetable research station, Anand Agricultural University, Anand, India.

DOI: 10.4236/ajmb.2013.34031                

Abstract

With the discovery of molecular markers and marker assisted selection technology, the research has entered into a new era and has made it possible to develop new and more informative PCR-based markers, including SSR, and to further facilitate the use of markers in tomato breeding. The present study is a step to introduce a new SSR marker (TOM-144) which was deduced after evaluation of eight microsatellite loci amongst the twenty-one different tomato cultivars. The marker selected was inherited and segregated in mendelian fashion as demonstrated in successive generation of a cross between parent cvs. H-24 x GT-2.

Keywords

Fusarium Wilt; Molecular Marker; Tomato

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Tanksley, S.D. and McCouch, S.R. (1997) Seed banks and molecular maps: Unlocking genetic potential from the wild, Science, 277, 1063-1066. http://dx.doi.org/10.1126/science.277.5329.1063
[2]	Foolad, M.R. and Panthee, D.R. (2012) Marker-assisted selection in tomato breeding. Critical Reviews in Plant Sciences, 31, 93-123. http://dx.doi.org/10.1080/07352689.2011.616057
[3]	Parmar, P.P., Oza, V.P., Patel, A.D., Kathiria, K.B. and Subramanian, R.B. (2011) Development of a rapid and reliable bioassay to discriminate between susceptible and resistant cultivars of tomato against Fusarium wilt. International Journal of Biosciences Agriculture and Technology, 3, 6-11.
[4]	Oza, V., Trivedi, S., Parmar, P. and Subramanian, R.B. (2008) A simple, rapid and efficient method for isolation of genomic DNA from plant tissue. Journal of Cell and Tissue Research, 8, 1383-1386.
[5]	Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular cloning: A laboratory manual, 2nd Edition, Cold Spring Harbor Lab. Press, New York.
[6]	Parmar, P., Gandhi, M. and Subramanian, R.B. (2012) Isolation and molecular characterization of avirulence gene from Indian isolates of Fusarium oxysporum f. sp. Lycopersici. Journal of Cell and Tissue Research, 12, 3061-3067. http://dx.doi.org/10.1139/g98-155
[7]	Areshchenkova, T. and Ganal, M.W. (1999) Long tomato microsatellites are predominantly associated with centromeric regions. Genome, 42, 536-544.
[8]	Suliman-Pollatschek, S., Kashkush, K., Shats, H. and Lavi, U. (2002) Generation and mapping of AFLP, SSRs and SNPs in Lycopersicon esculentum. Cellular Molecular Biology Letters, 7, 583-597.
[9]	Parmar, P., Bhatt, K., Oza, V., Kathiria, K. B. and Subramanan, R.B. (2009) Microsatellite marker associated with Fusarium wilt resistance in tomato. World Journal of Agricultural Sciences, 5, 389-393.
[10]	Lievens, B., Rep, M. and Thomma, B.P. (2008) Recent developments in the molecular discrimination of formae speciales of Fusarium oxysporum. Pest Management Science, 64, 781-788. http://dx.doi.org/10.1002/ps.1564
[11]	Marshall, T.C., Slate, J., Kruuk, L.E.B. and Pemberton, J.M. (1998) Statistical confidence for likelihood based paternity inference in natural populations. Molecular Ecology, 7, 639-655. http://dx.doi.org/10.1046/j.1365-294x.1998.00374.x
[12]	Dakin, E.E. and Avise, J.C. (2004) Microsatellite null alleles in parentage analysis. Heredity, 93, 504-509. http://dx.doi.org/10.1038/sj.hdy.6800545
[13]	Sobel, E., Papp, J.C. and Lange, K. (2002) Detection and integration of genotyping errors in statistical genetics. American Journal of Human Genetics, 70, 496-508. http://dx.doi.org/10.1086/338920
[14]	Michelmore, R.W., Paran, I. and Kesseli, R.V. (1991) Identification of markers linked to disease resistance genes by bulked segregation analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proceedings of the national academy of sciences, 88, 9828-9832. http://dx.doi.org/10.1073/pnas.88.21.9828
[15]	Eileen, K., Wencai, Y. and Francis, D.M. (2004). Improved tomato fruit color within an inbred backcross line derived from lycopersicion esculentum and L. hirsutum involves the interaction of loci. Journal of the American Society for Horticultural Science, 129, 250-257.
[16]	Cregan, P., Van Berkum, P., Sloger, C. and Orellana, R.G. (1989) Heterosis of interspecific soybean hybrids for traits related to N2 fixation. Euphytica, 40, 89-96.