Effect of Peaches, Pears and Green Tea on Plasma Lipids Profile and Antioxidant Content in Rats Fed High Sucrose Diet

Nada A. Al Zunaidy¹, Noorah S. Al-Sowayan^2*, Hassan M. Mousa³

¹Department of nutrition, College of Design and Home Economic, Qassim University, Qassim, Saudi Arabia.
²Department of Biology, Faculty of Science, Buraidah, Qassim University, Qassim, Saudi Arabia.
³Department of Food Science and Human Nutrition, College of Agriculture &Veterinary Medicine, Qassim University, Qassim, Saudi Arabia.

DOI: 10.4236/fns.2015.610094                

Abstract

The beneficial effects of feeding syrup of peaches and pears (peel and pulp) & green tea on the restoration of the changes produced by feeding high sucrose diet on plasma lipid profile, plasma antioxidant activity, and antioxidant enzymes in red blood cells (RBC) hemolysate were studied. High sucrose diet induced hyperlipidemia as manifested by significant (P ≤ 0.05) increases in the levels of cholesterol, triglycerides (TG) and low density lipoproteins (LDL) in plasma from 103.33 ± 1.8, 61.17 ± 0.48 and 26.6 ± 5.01 mg/dl to 136.67 ± 2.2, 81.3 ± 1.5 and 64.6 ± 6.5 mg/dl respectively. The high density lipoproteins (HDL) level was not significantly affected. TG levels in heart muscles increased significantly P ≤ 0.05 from the level of 194.14 ± 4.35 μg/g in the negative control to 269.72 ± 5.39 μg/g. Administration of fruits syrup and green tea resulted in a significant variable reduction in the elevated levels of cholesterol, TG and LDL and TG in the heart. Administration of sucrose resulted also in elevation of Malondialdehyde (MDA) in plasma from 5.06 ± 0.18 in the negative control to 14.81 ± 0.48 μmol/L in the positive control. This was also accompanied by reduction in the activities of supper oxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and a decrease in the concentration of reduced glutathione (GSH) in plasma, indicating lipid peroxidation. The activities of SOD, GSH-Px, CAT in the negative control were 8.96 ± 0.19, 315.67 ± 5.86, 209 ± 2.18 U/gHb respectively and were reduced significantly in the positive control to 4.7 ± 0.2, 273.83 ± 7.85, 207 ± 2.53 U/g Hb. There was a significant decrease in the level of total antioxidant status (TAS) in plasma from 1.45 ± 0.09 to 0.88 ± 0.09 nmol/L. However, no statistically significant changes were noticed in the concentrations of plasma total proteins and albumin. Intake of syrup of peaches and pears (peel and pulp) & green tea increased the level of TAS and GSH and decreased the level of LDL. The treatment also decreased significantly (P ≤ 0.05) the levels of plasma cholesterol, TG and heart TG levels. The activities of SOD and GSH-Px were increased following the treatment. It can be concluded that syrup of peaches and pears (peel and pulp) & green tea modulate lipid metabolism and enhance antioxidant status and in this respect green tea produced the best effect followed by peels of pears and peaches. The beneficial effect produced by fruits syrup and green tea may be due to their contents of phytochemicals.

Keywords

Sucrose, Lipid Profile, Lipid Peroxidation, Green Tea, Fruits

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Rekhy, R. and McConchie, R. (2014) Promoting Consumption of Fruit and Vegetables for Better Health. Have Campaigns Delivered on the Goals. Appetite, 79, 113-123. http://dx.doi.org/10.1016/j.appet.2014.04.012
[2]	Glasson, C., Chapman, K. and James, E. (2011) Fruit and Vegetables Should Be Targeted Separately in Health Promotion Programmes: Differences in Consumption Levels Barriers, Knowledge and Stages of Readiness for Change. Public Health Nutrition, 14, 694-701. http://dx.doi.org/10.1017/S1368980010001643
[3]	Halicka, E. and Rejman, K. (2007) Fruit and Vegetable Promotion Programs in the European Union. Problems of World Agriculture. Warsaw University of Life Sciences Press, Warsaw.
[4]	He, F.J., Nowson, C.A. and MacGregor, G.A. (2006) Fruit and Vegetable Consumption and Stroke: Meta-Analysis of Cohort Studies. The Lancet, 367, 320-326. http://dx.doi.org/10.1016/S0140-6736(06)68069-0
[5]	He, F.J., Nowson, C.A., Lucas, M. and MacGregor, G.A. (2007) Increased Consumption of Fruit and Vegetables Is Related to a Reduced Risk of Coronary Heart Disease: Meta-Analysis of Cohort Studies. Journal of Human Hypertension, 21, 717-728. http://dx.doi.org/10.1038/sj.jhh.1002212
[6]	Ortega, R.M. (2006) Importance of Functional Foods in the Mediterranean Diet. Public Health Nutrition, 9, 1136-1140. http://dx.doi.org/10.1017/s1368980007668530
[7]	Radhika, G., Sudha, V., Mohan Sathya, R., Ganesan, A. and Mohan, V. (2008) Association of Fruit and Vegetable Intake with Cardiovascular Risk Factors in Urban South Indians. The British Journal of Nutrition, 99, 398-405. http://dx.doi.org/10.1017/S0007114507803965
[8]	World Health Organization (2000) Obesity: Preventing and Managing the Global Epidemic: Report of a WHO Consultation. WHO Technical Report Series 894, World Health Organization, Geneva.
[9]	Wang, S., Melnyk, J.P., Tsao, R. and Marcone, M.F. (2011) How Natural Dietary Antioxidants in Fruits, Vegetables and Legumes Promote Vascular Health. Food Research International, 44, 14-22. http://dx.doi.org/10.1016/j.foodres.2010.09.028
[10]	Dauchet, L., Amouyet, P. and Dallongeville, J. (2005) Fruit and Vegetable Consumption and Risk of Stroke. A Meta-Analysis of Cohort Studies. Neurology, 65, 1193-1197. http://dx.doi.org/10.1212/01.wnl.0000180600.09719.53
[11]	Hu, F.B. (2003) Plant-Based Foods and Prevention of Cardiovascular Disease: An Overview. The American Journal of Clinical Nutrition, 78, 544S-551S.
[12]	Ness, A.R. and Powles, J.W. (1997) Fruit and Vegetables, and Cardiovascular Disease: A Review. International Journal of Epidemiology, 26, 1-13. http://dx.doi.org/10.1093/ije/26.1.1
[13]	Nothlings, U., Schulze, M.B., Weikert, C., Boeing, H., van der Schouw, Y.T. and Bamia, C. (2008) Intake of Vegetables, Legumes, and Fruit, and Risk for All-Cause Cardiovascular, and Cancer Mortality in a European Diabetic Population. The Journal of Nutrition, 138, 775-781.
[14]	Al-Nuaim, A. (1997) Effect of Overweight and Obesity on Glucose Intolerance and Dyslipidemia in Saudi Arabia, Epidemiological Study. Diabetes Research and Clinical Practice, 36, 181-191. http://dx.doi.org/10.1016/S0168-8227(97)00041-7
[15]	Al Balla, S., Bamgboye, E.A., Al Balla, S.R., Al Sekait, M. and Al Rasheed, R. (1993) Pattern of Adult Admission into Medical Wards of King Khalid University Hospital, Riyadh (1985-1990). Saudi Medical Journal, 13, 8-13.
[16]	Al Balla, S.R., Bamgboye, E.A., Sekait, M. and Balla, M. (1993) Causes of Morbidity in the Elderly Population of Saudi Arabia. The Journal of Tropical Medicine and Hygiene, 96, 157-162.
[17]	Al-Nuaim, A.R., AlRubeaan, K.A. and AlMazrou, Y. (1996) High Prevalence of Overweight and Obesity in Saudi Arabia. International Journal of Obesity, 20, 547-552.
[18]	Cullen, P. (2000) Evidence that Triglycerides Are an Independent Coronary Heart Disease Risk Factor. American Journal of Cardiology, 9, 86-943. http://dx.doi.org/10.1016/s0002-9149(00)01127-9
[19]	Haim, M., Benderly, M., Brunner, D., Behar, S., Graff, E., Reicher-Reiss, H. and Goldbourt, U. (1999) Elevated Serum Triglyceride Levels and Long-Term Mortality in Patients with Coronary Heart Disease. Circulation, 100, 475-482. http://dx.doi.org/10.1161/01.CIR.100.5.475
[20]	JBR (1998) Joint British Recommendations on Prevention of Coronary Heart Disease in Clinical Practice. Heart, 80, S1-S29.
[21]	Graham, H.N. (1992) Green Tea Composition, Consumption, and Polyphenol Chemistry. Preventive Medicine, 21, 334-350.
[22]	Sabu, M.C., Smitha, K. and Kuttan, R. (2002) Anti-Diabetic Activity of Green Tea Polyphenols and Their Role in Reducing Oxidative Stress in Experimental Diabetes. Journal of Ethnopharmacology, 83, 109-116.
[23]	Lin, J.-K., Lin, C.-L., Liang, Y.-C., Lin-Shiau, S.-Y. and Juan, I.-M. (1998) Survey of Catechins, Gallic Acid, and Methylxanthines in Green, Oolong Pu-erh, and Black Tea. Journal of Agricultural and Food Chemistry, 46, 3635-3642. http://dx.doi.org/10.1021/jf980223x
[24]	Lee, M-H., Chen, S.-C. and Min, B.-W. (1989) Effects of Extraction Method on the Determination of Tea Tannin and Catechins. Journal of Chinese Agricultural Chemistry, 27, 82-88.
[25]	Almajano, M.P., Carbo, R., Jiménez, J. and Gordon, M.H. (2008) Antioxidant and Antimicrobial Activities of Tea Infusions. Food Chemistry, 108, 55-63. http://dx.doi.org/10.1016/j.foodchem.2007.10.040
[26]	Weber, J.M., Ruzindana-Umunyana, A., Imbeault, L. and Sircar, S. (2003) Inhibition of Adenovirus Infection and Adenain by Green Tea Catechins. Antiviral Research, 58, 167-173. http://dx.doi.org/10.1016/S0166-3542(02)00212-7
[27]	Kono, S., Shinchi, K., Ikeda, N., Yanai, F. and Imanishi, K. (1992) Green Tea Consumption and Serum Lipid Profiles: A Cross-Sectional Study in Northern Kyushu, Japan. Preventive Medicine, 21, 526-531. http://dx.doi.org/10.1016/0091-7435(92)90060-U
[28]	Stensvold, I., Tverdal, A., Solvoll, K. and Foss, O.P. (1992) Tea Consumption Relationship to Cholesterol, Blood Pressure, and Coronary and Total Mortality. Preventive Medicine, 4, 546-553.
[29]	Muramatsu, K., Fukuyo, M. and Jara, Y. (1986) Effect of Green Tea Catechins on Plasma Cholesterol Level in Cholesterol-Fed Rats. Journal of Nutritional Science and Vitaminology, 32, 622-613. http://dx.doi.org/10.3177/jnsv.32.613
[30]	Kim, M.K., Cho, S.W. and Park, Y.K. (2007) Long-Term Vegetarians Have Low Oxidative Stress, Body Fat, and Cholesterol levels. Nutrition Research and Practice, 6, 155-161. http://dx.doi.org/10.4162/nrp.2012.6.2.155
[31]	Raja, M., Hernández-Revelles, J., Hernández-Cassou, S. and Saurina, J. (2014) Deter Mination of Polyphenols in the Pear Pulp Matrix by Solvent Extraction and Liquid Chromatography with UV-Vis Detection. Analytical Methods, 24, 9769-9776. http://dx.doi.org/10.1039/C4AY02558J
[32]	Amiot, M.J., Tacchini, M., Aubert, S.Y. and Oleszek, W. (1995) Influence of Cultivar, Maturity Stage, and Storage Conditions on Phenolic Composition and Enzymic Browning of Pear Fruits. Journal of Agricultural and Food Chemistry, 43, 1132-1137. http://dx.doi.org/10.1021/jf00053a004
[33]	Lin, L.Z. and Harnly, J.M. (2008) Phenolic Compounds and Chromatographic Profiles of Pear Skins (Pyrus spp.). Journal of Agricultural and Food Chemistry, 56, 9094-9101. http://dx.doi.org/10.1021/jf8013487
[34]	Subedi, P.P. and Walsh, K.B. (2009) Non-Invasive Techniques for Measurement of Fresh Fruit Firmness. Postharvest Biology and Technology, 51, 297-304. http://dx.doi.org/10.1016/j.postharvbio.2008.03.004
[35]	Tomás-Barberán, F.A., Gil, M.I., Cremin, P., Waterhouse, A.L., Hess-Pierce, B. and Kader, A.A. (2001) HPLC-DAD-ESIMS Analysis of Phenolic Compounds in Nectarines Peaches, and Plums. Journal of Agricultural and Food Chemistry, 49, 4748-4760. http://dx.doi.org/10.1021/jf0104681
[36]	Caballero, B. (2013) Sucrose: Dietary Sucrose and Disease. In: Caballero, B., Ed., Encyclopedia of Human Nutrition 3rd Edition, Elsvier, Amsterdam, 231-233. http://dx.doi.org/10.1016/B978-0-12-375083-9.00257-9
[37]	Lombardo, Y.B., Chicco, A., Mocchiuttti, N., de Rodi, M.A., Nusinmovich, B. and Gutman, R. (1983) Effect of Sucrose Diet on Insulin Secretion in Vivo and in Vitro and on Triglyceride Storage and Mobilization of the Heart of Rats. Hormone and Metabolic Research, 15, 69-76.
[38]	Lombardo, Y.B., Drago, S., Chicco, A., Fainstein-Day, P., Gutman, R., Gagliardino, J.J. and Gomez Dumm, C.L. (1996) Long-Term Administration of a Sucrose-Rich Diet to Normal Rats: Relationship Between Metabolic and Hormonal Profiles and Morphological Changes in the Endocrine Pancreas. Metabolism, 45, 1527-1532. http://dx.doi.org/10.1016/S0026-0495(96)90183-3
[39]	Rovenko, B.M., Kubrak, O.I., Gospodaryov, D.V., Perkhulyn, N.V., Yurkevych, I.S., Sanz, A., Lushchak, O.V. and Lushchak, V.I. (2015) High Sucrose Consumption Promotes Obesity whereas Its Low Consumption Induces Oxidative Stress in Drosophila Melanogaster. Journal of Insect Physiology, 79, 42-54. http://dx.doi.org/10.1016/j.jinsphys.2015.05.007
[40]	Lukaszewicz-Hussain, A. and Moniuszko-Jakoniuk, J. (2004) Liver Catalase, Glutathione Peroxidase and Reductase Activity, Reduced Glutathione and Hydrogen Peroxide Levels in Acute Intoxication with Chlorfenvinphos, an Organophosphate Insecticide. Polish Journal of Environmental Studies, 13, 303-309.
[41]	Reitman, S. and Frankel, S. (1957) A Colorimetric Method for Determination of Serum Glutamic Oxalacetic and Glutamic Pyruvic Transaminase. American Journal of Clinical Pathology, 28, 56-63.
[42]	Bradford, M.A. (1976) A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry, 72, 248-256. http://dx.doi.org/10.1016/0003-2697(76)90527-3
[43]	Tietz, N.W. (1987) Fundamentals of Clincial Chemistry. 3rd Edition, W.B. Saunders, Philadelphia.
[44]	Davidson, I. and Henry, J.B. (1974) Clinical Diagnosis by Laboratory Methods. 15th Edition, W.B. Saunders, Philadelphia.
[45]	Grillo, F., Izzo, C., Mazzotti, G. and Murador, E. (1981) Improved Method for Determination of High-Density-Lipo- protein Cholesterol II. Enzymic Determination of Cholesterol in High-Density Lipoprotein Fractions with a Sensitive Reagent. Clinical Chemistry, 27, 375-379.
[46]	Folch, J., Lees, M. and Sloane Stanley, G.H. (1957) A Simple Method for the Isolation and Purification of Total Lipides from Animal Tissues. The Journal of Biological Chemistry, 226, 497-509.
[47]	Cameron, N.E. and Cotter, M.A. (1994) The Relationship of Vascular Changes to Metabolic Factors in Diabetes Mellitus and Their Role in the Development of Peripheral Nerve Complications. Diabetes/Metabolism Reviews, 10, 189-224. http://dx.doi.org/10.1002/dmr.5610100302
[48]	Prior, R.L. and Cao, G. (2000) Antioxidant Phytochemicals in Fruits and Vegetables: Diet and Health Implications. HortScience, 35, 588-592.
[49]	Belitz, H.-D. and Grosch, W. (1999) Fruits and Fruit Products. In: Belitz, H.-D. and Grosch, W., Eds., Food Chemistry, Chap. 18, Springer, New York, 748-799. http://dx.doi.org/10.1007/978-3-662-07281-3_19
[50]	Vizzotto, M., Cisneros-Zevallos, L. and Byrne, D.H. (2007) Large Variation Found in the Phytochemical and Antioxidant Activity of Peach and Plum Germplasm. Journal of the American Society for Horticultural Science, 132, 334-340.
[51]	El-Razek, F.H.A. and Hassan, A.A. (2011) Nutritional Value and Hypoglycemic Effect of Prickly Cactus Pear (Opuntia ficus-indica) Fruit Juice in Alloxan-Induced Diabetic Rats. Australian Journal of Basic and Applied Sciences, 5, 356-377.
[52]	Gorinstein, S., Kulasek, G., Bartnikowska, E., Leontowicz, M., Morawiec, M., Zemser, M. and Trakhtenberg, S. (1998) The Influence of Persimmon Peel and Persimmon Pulp on the Lipid Metabolism and Antioxidant Activity of Rats Fed Cholesterol. The Journal of Nutritional Biochemistry, 9, 223-227. http://dx.doi.org/10.1016/S0955-2863(98)00003-5
[53]	Bocco, A., Cuvelier, M.E., Richard, H. and Berset, C. (1998) Antioxidant Activity and Phenolic Composition of Citrus Peel and Seed Extracts. Journal of Agricultural and Food Chemistry, 46, 2123-2129. http://dx.doi.org/10.1021/jf9709562