Polymerase Chain Reaction Detection of ASFV Infection in Nigerian Indigenous Pig

Olufunke O. Oluwole, Galman O. Omitogun

Faculty of Agriculture, Animal Science Department, Obafemi Awolowo University, Ile-Ife, Nigeria.
Institute of Agricultural Research and Training, Obafemi Awolowo University, Ile-Ife, Nigeria.

DOI: 10.4236/ajmb.2014.43017                

Abstract

This work investigated the presence of African Swine Fever Virus (ASFV) in Nigerian Indigenous Pig (NIP) using Polymerase Chain Reaction (PCR) screening method on the extracted DNA from the pig blood. NIP population selected from south western Nigeria were used for this study. ASFV infected blood samples collected from the University of Ibadan were used as positive control. Adiscrete and specific band was observed in both NIP and the infected samples show the presence of ASFV in NIP. The bands were of the expected size 278 base pairs (bp). The implication of this finding is that NIPs have domiciled ASFV without showing any clinical symptoms.

Keywords

Nigerian Indigenous Pig, Polymerase Chain Reaction, African Swine Fever

Conflicts of Interest

The authors declare no conflicts of interest.

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