A Fluorescence Ratiometric Probe for Cysteine/Homocysteine and Its Application for Living Cell Imaging


A fluorescence ratiometric probe 1 for cysteine (Cys) and homocysteine (Hcy) has been rationally constructed based on intramolecular charge transfer (ICT) mechanism. Upon treatment with Cys/Hcy, probe 1 exhibited a fluorescence ratiometric response, with the emission wavelength displaying a large shift (from 526 nm to 446 nm). When 90 μM Cys were added, the emission ratios (I446/I526) of the probe changed dramatically from 0.01797 to 4.65472. The detection limit was also measured to be 0.18 μM (S/N = 3). The theoretical calculations have confirmed that the ratiometric response of probe 1 to Cys/Hcy is due to the inhibition of ICT process upon the reaction of probe 1 with Cys/Hcy. Furthermore, the fluorescence imaging experiments in living cell demonstrated that probe 1 was favourable for intracellular Cys/Hcy imaging.

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L. Long, L. Wang and Y. Wu, "A Fluorescence Ratiometric Probe for Cysteine/Homocysteine and Its Application for Living Cell Imaging," International Journal of Organic Chemistry, Vol. 3 No. 4, 2013, pp. 235-239. doi: 10.4236/ijoc.2013.34033.

Conflicts of Interest

The authors declare no conflicts of interest.


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