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has been cited by the following article:

• TITLE: A Fluorescence Ratiometric Probe for Cysteine/Homocysteine and Its Application for Living Cell Imaging

  AUTHORS: Lingliang Long, Lin Wang, Yanjun Wu

  KEYWORDS: Fluorescent Probes; Fluorescence; Cysteine and Homocysteine; Fluorescence Imaging

  JOURNAL NAME: International Journal of Organic Chemistry, Vol.3 No.4, December 4, 2013

  ABSTRACT: A fluorescence ratiometric probe 1 for cysteine (Cys) and homocysteine (Hcy) has been rationally constructed based on intramolecular charge transfer (ICT) mechanism. Upon treatment with Cys/Hcy, probe 1 exhibited a fluorescence ratiometric response, with the emission wavelength displaying a large shift (from 526 nm to 446 nm). When 90 μM Cys were added, the emission ratios (I446/I526) of the probe changed dramatically from 0.01797 to 4.65472. The detection limit was also measured to be 0.18 μM (S/N = 3). The theoretical calculations have confirmed that the ratiometric response of probe 1 to Cys/Hcy is due to the inhibition of ICT process upon the reaction of probe 1 with Cys/Hcy. Furthermore, the fluorescence imaging experiments in living cell demonstrated that probe 1 was favourable for intracellular Cys/Hcy imaging.