Evaluation of Activation Energy and Thermodynamic Properties of Enzyme-Catalysed Transesterification Reactions
Ravindra Pogaku, Jegannathan Kenthorai Raman, Gujjula Ravikumar
DOI: 10.4236/aces.2012.21018   PDF    HTML     11,465 Downloads   21,801 Views   Citations


In this study, the activation energy and thermodynamic properties of immobilized enzyme catalysed transesterification reactions were evaluated based on the enzyme substrate transition theory. The activation energy for a enzyme catalysed biodiesel production system were found to be 4.25 (kcal/mole) for monoglyceride formation, 5.58(kcal/mole) for diglyceride formation and 5.50 (kcal/mole) for methyl ester formation respectively. The rate constants were found to be 3.2 × 1010(L/mol.sec) monoglyceride, 3.47 × 109 (L/mol.sec) for diglyceride and 3.93 × 109 (L/mol.sec) for methyl ester. Based on the present work and published literatures, the activation energy of enzyme-catalysed transesterification reactions were found to be lower than the chemical-catalysed and non-catalyzed transesterification reactions. The thermodynamic properties of immobilized enzyme-catalysed transesterification reaction were found to be Gibbs free energy (ΔG = –1.02 kcal/mol), enthalpy (ΔH = 544 cal/mol) and entropy (ΔS = 5.19 cal/Kmol).

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R. Pogaku, J. Raman and G. Ravikumar, "Evaluation of Activation Energy and Thermodynamic Properties of Enzyme-Catalysed Transesterification Reactions," Advances in Chemical Engineering and Science, Vol. 2 No. 1, 2012, pp. 150-154. doi: 10.4236/aces.2012.21018.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] M. Mittelbach, M. Worgetter, J. Pernkopf and H. Junek, “Diesel Fuel Derived from Vegetable Oils, II: Emission Tests Using Rape Oil Methyl Ester,” Energy in Agriculture, Vol. 4, 1985, pp. 207-215. doi:10.1016/0167-5826(85)90017-8
[2] L. C. Meher, D. V. Sagar and S. N. Naik, “Technical Aspects of Biodiesel Production by Transesterification—A Review,” Renewable and Sustainable Energy Reviews, Vol. 10, No. 3, 2006, pp. 248-268. doi:10.1016/j.rser.2004.09.002
[3] A. Demirbas, “Progress and Recent Trends in Biofuels,” Progress in Energy and Combustion Science, Vol. 33, No. 1, 2007, pp. 1-18. doi:10.1016/j.pecs.2006.06.001
[4] H. Fukuda, A. Kondo and H. J. Noda, “Biodiesel Fuel Production by Transesterification of Oils,” Journal of Bioscience and Bioengineering, Vol. 92, No. 5, 2001, pp 405-416. doi:10.1263/jbb.92.405
[5] K. R. Jegannathan, S. Abang, D. Poncelet, E. S. Chan and P. Ravindra, “Production of Biodiesel Using Immobilized Lipase—A Critical Review,” Critical Reviews in Biotechnology, Vol. 28, No. 4, 2008, pp. 253-264. doi:10.1080/07388550802428392
[6] Y. Xu, W. Du and D. Liu, “Study on Acyl Migration in Immobilized Lipozyme,” Journal of Molecular Catalysis B: Enzymatic, Vol. 32, No. 5-6, 2005, pp. 241-245. doi:10.1016/j.molcatb.2004.12.013
[7] V. Dossat, D. Combes and A. Marty, “Efficient Lipase Catalysed Production of a Lubricant and Surfactant Formulation Using a Continuous Solvent-Free Process,” Enzyme and Microbial Technology, Vol. 30, 2002, pp. 90-94. doi:10.1016/S0141-0229(01)00453-7
[8] S. Al-Zuhair, “Production of Biodiesel: Possibilities and Challenges,” Biotechnology Progress, Vol. 21, 2005, pp 1442-1448. doi:10.1021/bp050195k
[9] S. Al-Zuhair, Y. W. Fan and S. J. Lim, “Production of Biodiesel Using Immobilized Lipase—A Critical Review,” Biotechnology Progress, Vol. 42, 2007, pp. 951- 960.
[10] S. F. A. Halim, A. H. Kamaruddin and W. J. N. Fernando, “Continuous Biosynthesis of Biodiesel from Waste Cooking Palm Oil in a Packed Bed Reactor: Optimization Using Response Surface Methodology (RSM) and Mass Transfer Studies,” Bioresource Technology, Vol. 100, 2009, pp. 710-716. doi:10.1016/j.biortech.2008.07.031
[11] B. Cheirsilp and A. H. Kittikun, “Limkatanyu, Impact of Transesterification Mechanisms on the Kinetic Modeling of Biodiesel Production by Immobilized Lipase,” Biochemical Engineering Journal, Vol. 35, 2007, pp. 71-80. doi:10.1016/j.bej.2006.12.024
[12] S. F. A Halim and A. H. Kamaruddin, “Continuous Biosynthesis of Biodiesel from Waste Cooking Palm Oil in Packed Bed Reactor,” Process Biochemistry, Vol. 43, 2008, pp. 1436-1439. doi:10.1016/j.procbio.2008.08.010
[13] K. R. Jegannathan, E. S. Chan and P. J. Ravindra, “Design an Immobilized Lipase Enzyme for Biodiesel Production,” Journal of Molecular Catalysis B: Enzymatic, Vol. 58, 2009, pp. 78-83. doi:10.1016/j.molcatb.2008.11.009
[14] A. Liese, K. Seelbach and C. Wandrey, “Industrial Biotransformations,” Wiley-VCH Verlag GmbH & Co., Weinheim, 2006.
[15] A. F. Chang and Y. A. Liu, “Integrated Process Modeling and Product Design of Biodiesel Manufacturing,” Industrial & Engineering Chemistry Research, Vol. 49, No. 3, 2010, pp. 1197-1213. doi:10.1021/ie9010047
[16] J. Joelianingsih, H. Meada, S. Hagiwara, H. Nabetani, Y. Sagara, T. H. Soerawidjaya, A. H. Tambunan and K. Abdullah, “Biodiesel Fuels from Palm Oil via the Noncatalytic Transesterification in a Bubble Column Reactor at Atmospheric Pressure: A Kinetic Study,” Renewable Energy, Vol. 33, No. 7, 2008, pp. 1629-1636. doi:10.1016/j.renene.2007.08.011

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