Colour Removal from Aqueous Solutions of the Reactive Azo Dye Remazol Black B Using the Immobilised Cells (Shewanella Strain J18 143) – System

DOI: 10.4236/jwarp.2010.21009   PDF   HTML     9,434 Downloads   15,887 Views   Citations


Consideration is given here to colour removal, carried out using immobilised biological cells, Shewanella strain J18 143. In order to provide greater control of an overall colour removal process and to give a basis for the effective recovery of the cell culture species, cell immobilisation has been established on chemically modified cellulose. The modification was achieved by chemically inducing the graft copolymerisation of methacrylic acid onto cotton fabric. The immobilised cells were able to decolorise the dye. The immobilisation methods, physical adsorption, “growing-in” and chemical coupling, were compared. Each of the methods was effective to some extent. However, the latter two immobilisation methods provided the greater effect in decoloration. Each of these immobilised systems is relatively simple to achieve, whether by adsorption, physical interlocking or covalent coupling. The graft copolymer is able to offer versatility in use. The decoloration was shown to be rapid under relatively simple processing conditions. Thus, compared with the established controls, complete decoloration of solutions of Remazol Black B was observed. The potential use of the graft copolymer substrate as support for a biochemical agent was confirmed.

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T. LI and J. GUTHRIE, "Colour Removal from Aqueous Solutions of the Reactive Azo Dye Remazol Black B Using the Immobilised Cells (Shewanella Strain J18 143) – System," Journal of Water Resource and Protection, Vol. 2 No. 1, 2010, pp. 77-84. doi: 10.4236/jwarp.2010.21009.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] K. N. Tapley, “Dyes and pigment properties and applications,” Polymer Science Lecture, Department of Colour and Polymer Chemistry, UK: University of Leeds, 2002.
[2] J. García-Monta?o, N. Ruiz, I. Mu?oz, X. Domènech, J. A. Gracía-Hortal, F. Torrades, and J. Peral, “Environmental assessment of different photo-fenton approaches for commercial reactive dye removal,” Journal of Hazardous Materials, Vol. 138, pp. 218–225, November 2006.
[3] A. J. Smith, “Colour removal from dyehouse effluents,” Ph.D. thesis, University of Sheffield, UK, 2001.
[4] Y. M. Slokar and A. M. Le Marechal, “Methods of decoloration of textile wastewaters,” Dyes and Pigments, Vol. 37, No. 4, pp. 335–356, May 1998.
[5] C. I. Pearce, R. Christie, C. Boothman, H. Canstein, J. T. Guthrie, and J. R. Lloyd, “Reactive azo dye reduction by Shewanella strain J18 143,” Biotechnology and Bioengineering, Vol. 95, pp. 692–703, June 2006.
[6] T. H. Wallace, “Biological treatment of a synthetic dye water and an industrial textile wastewater containing azo dye compounds,” Masters Dissertation, Virginia Polytechnic Institute and State University, USA, 2001.
[7] C. I. Pearce, J. R. Lloyd, and J. T. Guthrie, “The removal of colour from textile wastewater using whole bacterial cells: a review,” Dyes and Pigments, Vol. 58, pp. 179– 196, September 2003.
[8] M. I. Banat, P. Nigham, D. Singh, and R. Marchant, “Microbial decolorization of textile dyes containing effluents: A review,” Bioresource Technology, Vol. 58, pp. 217– 227, December 1996.
[9] A. Stolz, “Basic and applied aspects in the microbial degradation of azo dyes,” Applied Microbiology and Biotechnology, Vol. 56, No. 1–2, pp. 69–80, July 2001.
[10] M. A. Brown and S. C. De Vito, “Predicting azo dye toxicity,” Ccritical Reviews in Environmental Science and Technology, Vol. 23, No. 3, pp. 249–324, 1993.
[11] C. T. M. J. Frijters, R. H. Vos, G. Scheffer, and R. Mulder, “Decolorizing and detoxifying textile wastewater, containing both solution and insoluble dyes, in a full scale combined anaerobic/aerobic system,” Water Research, Vol. 40, No. 6, pp. 1249–1257, March 2006.
[12] A. Gottlieb, C. Shaw, A. Smith, A. Wheatley, and S. Forsythe, “The toxicity of textile reactive azo dyes after hydrolysis and decolorisation,” Journal of Biotechnology, Vol. 101, No. 1, pp. 49–56, February 2003.
[13] E. Razo-Flores, M. Luijten, B. A. Donlon, G. Lettinga, and J. A. Field, “Complete biodegradation of the azo dye azodisalycilate under anaerobic conditions,” Environmental Science and Technology, Vol. 31, No. 7, pp. 2098–2103, 1997.
[14] D. T. Sponza and M. I?ik, “Decolorization and azo dye degradation by anaerobic/aerobic sequential process,” Enzyme and Microbial Technology, Vol. 31, No. 1–2, pp. 102–110, July 2002.
[15] N. J. Willmott, “The use of bacteria-polymer composites for the removal of colour from reactive dye effluents,” Ph.D. thesis, University of Leeds, UK, 1997.
[16] D. A. Saffarini, T. J. DiChristina, D. Bermudes, and K. H. Nealson, “Anaerobic respiration of Shewanella putrefaciens requires both chromosomal and plasmid-borne genes,” FEMS Microbiology Letters, Vol. 119, No. 3, pp. 271–278, January 1994.
[17] I. Chibata and T. Tosa, “Immobilized cells: Historical background,” Applied Biochemistry Bioengineering, Vol. 4, pp. 1–9, 1983.
[18] M. H. M. Gil, “Immobilisation of proteins, enzymes and cells onto graft copolymeric substrates,” Ph.D. thesis, University of Leeds, UK, 1983.
[19] A. Kamilaki, “The removal of reactive dyes from textile effluents - a bioreactor approach employing whole bacterial cells,” Ph.D. thesis, University of Leeds, UK, 2000.
[20] D. Roy, S. Perrier, and J. T. Guthrie, “RAFT graft copolymerisation of 2-(dimethylanimoethyl) methacrylate onto cellulose fibres,” Australian Journal of Chemsitry, Vol. 59, pp. 737–741, 2006.
[21] D. Roy, S. Perrier, and J. T. Guthrie, “Synthesis of natural-synthetic hybrid materials from cellulose via the RAFT process,” Soft Matter, Vol. 4, pp. 145–154, 2008.
[22] T. Li, “Removal of colour from solutions of azo dyes using bacterial cells (Shewanella Strain J18 143),” Ph.D. thesis, University of Leeds, UK, 2007.
[23] D. Roy, J. Knapp, S. Perrier, and J. T. Guthrie, “Antimicrobial cellulose fibres via RAFT surface graft polymerisation,” Biomacromolecules, Vol. 9, pp. 91–99, 2008.
[24] C. I. Pearce, “The reduction of coloured compounds using whole bacterial cells (Shewanella strain J18 143),” Ph.D. thesis, University of Leeds, UK, 2004.
[25] A. Hebeish and J. T. Guthrie, “The Chemistry and technology of cellulosic copolymers,” Springer-Verlag, Heidelberg, ISBN 3-540-10164-0, 1981.
[26] A. Carlmark and E Malmstrom, Journal of the American Chemical Society, Vol. 124, pp. 900–901, 2002.
[27] D. Roy, J. T. Guthrie, and S.Perrier, Australian Journal of Chemistry, Vol. 59, pp. 737–741, 2006.
[28] M. Sahnoun, M. T. Charrayre, L. Vernon, T. Delait, and F. D’Agosto, Journal of Polymer Science, Part A-Polymer Chemistry, Vol. 43, pp. 3551–3565, 2005.
[29] N. M. Mahmoodi, M. Arami, N. Y. Limaee, and N.S.Tabrizi, Chemical Engineering Journal, Vol. 112, pp. 191–196, 2005.
[30] J. L. Long, H. D. Stensel, J. F. Ferguson, S. E. Strand, and J. E. Ongerth, Journal of Environmental Engineering, Vol. 119, pp. 300–320, 1993.
[31] M. Xu, J. Guo, G. Zeng, and X. Zhong, Applied Microbiology and Biotechnology, Vol. 71, pp. 246–251, 2006.
[32] P. K. Smith, R. I. Krohn, G. T. Hermanson, A. K. Mallia, F. H. Gartner, M. D. Provenzano, E. K. Fujimoto, N. M. Goeke, B. J. Olson, and D. C. Klenk, “Measurement of protein using binchiconinic acid,” Analytical Biochemistry, Vol. 150, pp. 76–85, 1985.

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