Author(s)

Wendkouni Leila Marie Esther Belem-Kabré^1,2*, Vincent Ouédraogo², Bagora Bayala³, Alimata Bancé^1,2, Estelle Ouédraogo³, Boubacar Yaro¹, Lazare Belemnaba¹, Moussa Compaoré², Martin Kiendrébeogo², Noufou Ouédraogo^1,2,4

¹Department of Traditional Medicine and Pharmacopoeia-Pharmacy, Health Sciences Research Institute, Ouagadougou, Burkina Faso.
²Laboratory of Biochemistry and Applied Chemistry (LABIOCA), Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso.
³Laboratory of Molecular Biology and Genetics (LABIOGENE), Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso.
⁴International Research Laboratory: Environnement, Santé, Sociétés (IRL 3189, ESS) CNRST/CNRS/UCAD/UGB/USTTB, Ouagadougou, Burkina Faso.

Objective: This study focused on the antibacterial and anti-proliferative activity of extracts from Carica papaya and Cocos nucifera roots. Methodology: The minimum inhibitory concentration and the minimum bactericidal concentration of the extracts on Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, and Staphylococcus aureus were deduced by the microdilution method. The anti-biofilm activity was determined on all four strains and anti-quorum sensing activity by inhibition of violacein production in Chromobacterium violaceum. Anti-proliferative activity on prostate cultured cancer cells was evaluated by MTT assay. Sterols and triterpenes were also assayed in this study. Results: The methanolic extract of Carica papaya showed the best anti-biofilm effect with a percentage inhibition of 66.10 ± 1.79. The methanolic extract of Cocos nucifera had the strongest inhibition on the production of quorum sensing (61.42 ± 0.28). In addition, the methanolic extract of Cocos nucifera roots showed the best cytotoxic effect on prostate cancer LNCaP cell lines (IC₅₀ = 26.98 ± 2.6 μg/mL) and Carica papaya on the PC-3 lines (IC₅₀ = 127.20 ± 5.99 μg/mL). The extracts were also rich in triterpenes and sterols. Conclusion: This study provides support for the ethnomedical use of Carica papaya and Cocos nucifera roots as an antimicrobial and anticancer.

Triterpenes and Sterols Content, Antibacterial, Biofilm, Quorum Sensing, Anti-Proliferative, Medicinal Plants

Belem-Kabré, W. , Ouédraogo, V. , Bayala, B. , Bancé, A. , Ouédraogo, E. , Yaro, B. , Belemnaba, L. , Compaoré, M. , Kiendrébeogo, M. and Ouédraogo, N. (2023) Anti-Biofilm, Anti-Quorum Sensing, and Anti-Proliferative Activities of Methanolic and Aqueous Roots Extracts of Carica papaya L. and Cocos nucifera L.. Advances in Microbiology, 13, 165-180. doi: 10.4236/aim.2023.134010.