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has been cited by the following article:

• TITLE: The Advantages of Methane Production by Combined Fermentation of Lignite and Wheat Straw

  AUTHORS: Jiayuan Gu

  KEYWORDS: Lignite, Wheat Straw, Mixed Fermentation, Microbial Community, Macrogenomics

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.15 No.1, January 15, 2024

  ABSTRACT: Biogasification of coal is important for clean utilization of coal. Experiments on the fermentation of single lignite, single straw and their mixture were performed to explore the variation characteristics of gas production potential, microbial community and methanogenic metabolic pathways of mixture. Research has shown that mixed fermentation of lignite and straw significantly promoted biomethane production. The abundance of hydrolytic acidifying functional bacteria genera (Sphaerochaeta, Lentimicrobium) in mixed fermentation was higher than that in the fermentation of single lignite and single straw. The abundance of some key CAZy metabolic enzyme gene sequences in mixed fermentation group was increased, which was favorable to improve methane production. Aceticlastic methanogenesis was the most critical methanogenic pathway and acetic acid pathway was more competitive in methanogenic mode during peak fermentation. Macrogenomics provided theoretical support for the claim that mixed fermentation of coal and straw promoted biomethane metabolism, which was potentially valuable in expanding methanogenesis from mixed fermentation of lignite with different biomasses.