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has been cited by the following article:

• TITLE: Temperature and Pressure Behaviours of Methanol, Acetonitrile/Water Mixtures on Chromatographic Systems

  AUTHORS: Talal Aburjai, Muhammed Alzweiri, Yusuf M. Al-Hiari

  KEYWORDS: Retention Time, Pressure, Temperature, Methanol and Acetonitrile

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.2 No.8, December 8, 2011

  ABSTRACT: Temperature and pressure were shown to vary significantly with solvent mixing, showing maxima at differ-ent solvent ratios. Acetonitrile/water mixing resulted in temperature reduction of solutions whereas metha-nol/water mixing caused temperature increases. On the other hand, maximum recorded chromatographic pressure of acetonitrile:water mixtures occurred at a solvent ratio of 1:6 compared with methanol:water, which showed a maximum pressure at a solvent ratio of 1:1. These findings can be of use in stabilizing re-tention time shifts during HPLC-based studies associated with compound identification based on retention time such as analysis of complex mixtures.