Temperature and Pressure Behaviours of Methanol, Acetonitrile/Water Mixtures on Chromatographic Systems

Talal Aburjai, Muhammed Alzweiri, Yusuf M. Al-Hiari

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DOI: 10.4236/ajac.2011.28108                

Abstract

Temperature and pressure were shown to vary significantly with solvent mixing, showing maxima at differ-ent solvent ratios. Acetonitrile/water mixing resulted in temperature reduction of solutions whereas metha-nol/water mixing caused temperature increases. On the other hand, maximum recorded chromatographic pressure of acetonitrile:water mixtures occurred at a solvent ratio of 1:6 compared with methanol:water, which showed a maximum pressure at a solvent ratio of 1:1. These findings can be of use in stabilizing re-tention time shifts during HPLC-based studies associated with compound identification based on retention time such as analysis of complex mixtures.

Keywords

Retention Time, Pressure, Temperature, Methanol and Acetonitrile

Conflicts of Interest

The authors declare no conflicts of interest.

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