TITLE:
Novel Approach to Microbiological Study of Chronic Inflammations at Upper Respiratory Tract: Research of Blood L-Form Microbiota
AUTHORS:
Nadya Markova
KEYWORDS:
L-Forms, Blood Microbiota, Chronic Inflammation, Upper Respiratory Tract
JOURNAL NAME:
Open Journal of Medical Microbiology,
Vol.11 No.3,
August
6,
2021
ABSTRACT: Background: The recognition of human blood microbiota, consisting of cell
wall-deficient microbes (L-forms), is a major challenge today in the field of
microbiology. There are accumulating data confirming the concept of “internal”
blood L-form microbiota and its significance for health and diseases. Finding
out whether the blood microbiota can be of diagnostic and prognostic importance
for detection and evaluation of chronic infections anywhere in the body is a major objective. In the context of
chronically infected upper respiratory
tract (URT), the aim of the current study was to understand whether a local infection can be a source for entry of
bacteria and fungi in the blood. Methods: Blood samples from six persons
with chronic inflammations in URT diagnosed with hypertrophied adenoids,
chronic sinusitis, nasal polyps, chronic naso-pharyngitis and one control
healthy person were studied. Blood microbiota assessment methodology that be
used, included three phases: 1) isolation of L-form cultures from blood-development
and propagation; 2) cultivation directed to conversion of L-forms into bacterial and fungal
cultures; 3) isolation of pure classical bacterial and fungal
cultures and their identification
by MALDI-TOF method. Results: From the patients were isolated L-forms
of opportunistic bacteria (Streptococcus
mitis, Roseomonas mucosa, Dermacoccus nishinomiyaensis, Enterococcus faecalis, Acinetobacter johnsonii, Pseudomonas putida, Staphylococcus aureus, Pseudomonas luteola, Enterobacter
cloacae) and fungi such as Rhodotorula
mucilaginosa, Aspergillus niger, Aspergillus fumigatus and Mucorales. Conclusion: The novel innovative methodology for assessment of blood L-form microbiota was
successfully applied for detection of microbes responsible for chronic
infections at URT.