TITLE:
A salt, detergent, and solvent tolerant protease from Bacillus sp. B001: Low-cost, easy-purified, and enhanced production by raw material based culture strategy
AUTHORS:
Jie Wu, Aihua Deng, Nana Shi, Shuwen Liu, Yong Liang, Tingyi Wen
KEYWORDS:
Alkaline Protease; Bacillus; Enzyme Production; Raw Materials Based Strategy; Response Surface Methodology
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.12,
December
23,
2013
ABSTRACT:
To enhance the protease production and decrease cost, corn flour and soy peptone were screened as cheap raw materials for the production of extracellular proteases by Bacillus strains. Their compositions in the medium suitable for enzyme production of Bacillus sp. B001 were optimized using statistical experiment designs. Under the optimized conditions, the protease production of Bacillus sp. B001 was stable at the stationary stage and reached to 63,200 U/mL, approximately 1.84-fold increase compared with that using the original medium. These improvements could be attributed to the release of the catabolite repression by crude materials corn flour and soy peptone which contained low level of available nutrients. Additionally, a highly pure protease which displayed excellent stability and compatibility with high salinity, commercial laundry detergents, and organic solvents, was rapidly obtained by two-step procedure involving ammonium sulphate precipitation and anion exchange from the fermentation cultures of B001 in the optimized medium. When the culture method applied to other Bacillus strains, their protease yields were all remarkably increased approximately 2.9 to 8.5 folds. In conclusion, a low-cost, easy-purified, and effective producing strategy using the cheap raw materials was developed here, representing a potential application for protease production in various Industrial processes.