M. Yasuda, K. Takeo, T. Matsumoto, T. Shiragami, Y. Matsushita, K. Sugamoto and Y. Ishii, “Effectiveness of Lignin-Removal in Simultaneous Saccharification and Fermentation of Napiergrass, Rice Straw, Silbergrass and Bamboo with Different Lignin-Contents,” In: A. K. Chandel and S. Silverio da Silva, Eds., Sustainable Degradation of Lignocellulosic Biomass—Techniques, Applications and Commercialization, InTech, Croatia, Chapter 4, 2013, pp. 91-104.

has been cited by the following article:

• TITLE: Enhancement of Ethanol Production from Napiergrass (Pennisetum purpureum Schumach) by a Low-Moisture Anhydrous Ammonia Pretreatment

  AUTHORS: Masahide Yasuda, Keisuke Takeo, Hayato Nagai, Takuya Uto, Toshifumi Yui, Tomoko Matsumoto, Yasuyuki Ishii, Kazuyoshi Ohta

  KEYWORDS: LMAA; SSF; Cellulase; Xylanase; Saccharomyces cerevisiae; Escherichia coli KO11

  JOURNAL NAME: Journal of Sustainable Bioenergy Systems, Vol.3 No.3, September 13, 2013

  ABSTRACT: Napiegrass (Pennisetum purpureum Schumach) was treated with a low-moisture anhydrous ammonia (LMAA) pretreatment by adding an equal weight of water and keeping it under atmospheric ammonia gas at room temperature for four weeks. After the removal of ammonia and washing with water, a simultaneous saccharification and fermentation (SSF) was conducted for the LMAA-pretreated napiergrass (1.33 g) in a buffer solution (8 mL) using a mixture of a cellulase (80 mg) and a xylanase (53 mg) as well as the cell suspension (0.16 mL) of Saccharomyces cerevisiae. Ethanol and xylose resulted in 91.2% and 62.9% yields, respectively. The SSF process was scaled up using LMAA-pretreated napiergrass (100.0 g) to give ethanol (77.2%) and xylose (52.8%). After the removal of ethanol, the pentose fermentation of the SSF solution (40 mL), which contained 1.00 g of xylose, using cell suspension of Escherichia coli KO11 (70 mL) gave 86.3% yield of ethanol. Total ethanol yield reached 68.9% based on xylan (21.4 wt%) and glucan (39.7 wt%) of the LMAA-pretreated napiergrass.