TITLE:
Protocol for maximizing the triglycerides-enriched lipids production from Dunaliella salina SA32007 biomass, isolated from the Salar de Atacama (Northern Chile)
AUTHORS:
Diana Arias-Forero, Gladys Hayashida, Mario Aranda, Solange Araya, Tamara Portilla, Andreína García, Paula Díaz-Palma
KEYWORDS:
Lipids; Triacilglycerides; Nitrogen Deficiency; Salinity; Dunaliella salina
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.8,
August
1,
2013
ABSTRACT:
This paper reports the effects of different culture conditions for Dunaliella salina SA32007 from Salar de Atacama (second Region, northern ofChile) over
biomass, lipid production and triglycerides synthesis. A maximum value of
microalgae density (8.2 × 109 Cells/L) and an intrinsic growth rate (0.17 d-1),
were obtained using a culture with 0.5 mol/L of NaCl and a nitrogen/phosphorous
(N/P) limitation of 14/1. The triglycerides production was significantly
favoured under nitrogen deficiency conditions (Mann-Whitney test; p = 0.0043).
However there was a nitrogen-limiting threshold for the stimulation and
accumulation of triglycerides (N/P: 14/1), lower than that limit, these
compounds would not be accumulated. It was also observed that triglyceride
content was not proportional to the total lipid content and the maximum number
of cells. The aeration system employed stimulated the growth and synthesis of
structural organic molecules. Regarding microalgae growth stage subjected to
nitrogen deficiency, when the deficit was applied before the lag phase, the
negative effect on the biomass and the triglycerides production decreased.