TITLE:
Protecting effect of He-Ne laser on winter wheat from UV-B radiation damage by analyzing proteomic changes in leaves
AUTHORS:
Zhenhu Jia, Jiangyan Duan
KEYWORDS:
Winter Wheat; Protein; Ultraviolet-B Radiation; He-Ne Laser
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.8,
August
1,
2013
ABSTRACT:
The ultraviolet-B (UV-B) radiation can affect gene expression of
plant in growth and development. Winter wheat (Triticum
aestivum), as a kind of
economic crop cultured in the Northern China, is also damaged by present-day
ultraviolet-B (UV-B) radiation. It was reported that He-Ne lasers could
alleviate cell damage caused by UV-B radiation in plants. To get the proteomic
changes of wheat alleviated by He-Ne lasers, we studied protein expression
profiles of winter wheat (Jin Mai NO.79) leaves by running 2-DE (two-dimensional gel
electrophoresis), which allowed the identification of some significantly
different gel spots. The spots were further verified by matrix-assisted laser
desorption/ionization-time of flight mass spectrometry, in which they were
confirmed to be winter wheat proteins. The results showed: 1) the proteomic
changes between the ultraviolet-B radiation group and normal light group were
significantly different on sixth day; 2) The expression of some proteins such
as catalase, DNA polymerase and reduced glutathione declines at the
ultraviolet-B radiation group and increases at the UV-B + He-Ne laser group.
This indicates UV-B could regulate genes encoding proteins in Winter wheat
leaves and affect the growth of the Winter wheat. He-Ne lasers treatment could
significantly alleviate UV-B-induced damage in wheat.