TITLE:
A sensitive, rapid and validated liquid chromatography ? tandem mass spectrometry (LC-MS-MS) method for determination of Mimosine in Mimosa pudica Linn
AUTHORS:
Parikshit A. Champanerkar, Vikas V. Vaidya, Sunita Shailajan, Sasikumar N. Menon
KEYWORDS:
LC-MS-MS; Mimosine; Mimosa pudica Linn
JOURNAL NAME:
Natural Science,
Vol.2 No.7,
July
29,
2010
ABSTRACT: A rapid, sensitive and accurate liquid chroma-tographic tandem mass spectrometric method is described for the determination of Mimosine in Mimosa pudica Linn. whole plant powder. Mi-mosine was extracted from the plant using 1.0% HCl in water. The chromatographic separation was achieved using a Thermo Hypurity C18 (50 x 4.6 mm) 5.0 μ column interfaced with a triple quadrapole mass spectrometer. The mobile phase consisted of a mixture of Methanol: 10 mM ammonium formate buffer whose pH was ad-justed to 3.00 ± 0.05 with formic acid (80:20, v/v) and was delivered at a flow rate of 1.0 mL min-1. Electrospray ionization (ESI) source operated in the negative ion mode was used for the quantitation. Detection was performed using an Applied Biosystems Sciex API 3200 Mass spec-trometer. The method was found to be simple, precise, accurate, fast, specific and sensitive and can be used for routine quality control analysis of Mimosine in Mimosa pudica Linn.