TITLE:
Molecular Characterization of Pseudomonas spp. Isolated from Root Nodules of Various Leguminous Plants of Shekhawati Region, Rajasthan, India
AUTHORS:
Sakshi Issar, Saroj Sharma, Devendra Kumar Choudhary, Hemant Kumar Gautam, R. K. Gaur
KEYWORDS:
PGPRs; 16S Sequencing; Pseudomonas; Root-Nodule Bacteria; 16S rDNA
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.3 No.1,
December
31,
2011
ABSTRACT: Plant growth promontory Pseudomonas strains were isolated from root nodules of five plant species, viz., Trifolium pretense, Cicer arietinum, Amaranthus polygamus, Vigna mungo, and Trigonella foenum; that plants were denizen of Shekhawati region of Rajasthan. A total of 8 bacterial isolates were evaluated for growth promotion using PGP properties. Partial 16S rDNA sequencing data showed that these 8 bacterial isolates belonged to genus Pseudomonas. MEGA 4.0.2, software was used to construct a neighbor joining tree by employing boot strap method. Result exhibited significant diversity among recovered Pseudomonas strains.