M. Blaszczyk-Thurin, J. Thurin, O. Hindsgaul, K. A. Karlsson, Z. Steplewski and H. Koprowski, “Y and Blood Group B Type 2 Glycolipid Antigens Accumulate in a Human Gastric Carcinoma Cell Line as Detected by Monoclonal Antibody. Isolation and Characterization by Mass Spectrometry and NMR Spectroscopy,” Journal of Biological Chemistry, Vol. 262, No. 1, 1987, pp. 372-379.

has been cited by the following article:

• TITLE: Phase I Dose Escalation Study with the Lewis Y Carbohydrate Specific Humanized Antibody IGN311

  AUTHORS: Daniel Oruzio, Günter Waxenecker, Christoph Aulmann, Bruno Märkl, Theodor Wagner, Geert Mudde, Manfred Schuster, Norbert Eller, Andrea Mayer, Stefan Stranner, Gottfried Himmler, Hans Loibner, Günter Schlimok, Ralf Kircheis, Andreas Nechansky

  KEYWORDS: Passive Immunotherapy, Therapeutic Monoclonal Antibody, Disseminated Tumor Cells, Phase I Study, Lewis Y Carbohydrate, HAHA (Human Anti-Human Antibodies)

  JOURNAL NAME: Journal of Cancer Therapy, Vol.2 No.5, December 31, 2011

  ABSTRACT: Purpose: Investigation of safety, tolerability, pharmacokinetics, and anti-tumor activity of the Lewis Y-specific, fully humanized monoclonal antibody (mAb) IGN311 in patients with Lewis Y positive tumors in a Phase I clinical trial. Experimental Design: Twelve patients (pts) were enrolled in an open-label, uncontrolled, dose escalating Phase I study. Three pts received 50 mg, three pts 100 mg and six pts 200 mg IGN311 by i.v. infusion on days 1 and 15. Blood samples were taken immediately before infusion, and 0.5, 4, 8, 24 hours post infusion, as well as on days 3, 5 and 8 after the first and second infusion, respectively, and day 29. A final visit was scheduled for day 43. Results: No drug related adverse events were observed in the 50 mg and 100 mg dose groups. Three out of six patients in the 200 mg dose group showed drug related adverse reactions with nausea, vomiting and hypotension in one patient (NCI CTC grade 3) being the dose limiting toxicities. t1/2 of IGN311 was ~20 days after second infusion of IGN311. Sera of patients receiving IGN311 were capable of lysing Lewis Y positive tumor cells in vitro by both, complement-dependent cytotoxicity (CDC) and antibody-dependent cellular cytotoxicity (ADCC). Circulating tumor cells found in the peripheral blood in two out of twelve pts prior to treatment were reduced after treatment to below the quantification limit of the detection method. None of the patients showed an increase in the number of disseminated tumor cells during treatment period. Conclusions: The good safety and PK profile, the biological activity regarding CDC and ADCC mediated tumor cell lysis, and the elimination of circulating tumor cells warrant further clinical investigation of IGN311.