TITLE:
Synthesis, Characterization of Ruthenium Compounds and Studies of Biological Effects in MCF-7 Tumors Cell Lines
AUTHORS:
Andressa Teixeira de Barros Nunes Ribeiro, Claudia Cristina Sousa, Adenilda Cristina Honório França, Eduardo Luzia França, Fabricio Tarso Moraes, Wagner Batista Santos
KEYWORDS:
Ruthenium, MCF-7 Cells, Cytotoxic Evaluation, N-Heterocyclic Ligands
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.12 No.3,
June
30,
2022
ABSTRACT: This work presents the synthesis and characterization of compounds
derived from the ruthenium transition metal with the nitrogenous ligand
4-aminopy- ridine (4-ampy). The
synthesized compounds were characterized by FTIRmed spectroscopy and TG-DTA thermal analysis. For the
cytotoxic evaluation of ruthenium compounds, a 66.0 μM aqueous solution
containing the complex and the study of data observed in the biological
assessment was performed using variance (ANOVA) analysis, followed by Tukey’s
multiple comparisons test. Differences between treatments were considered
significant when the p-value was less than 0.05 (p complex suggests a
stoichiometry of [Ru(Cl)3(4-ampy)(H2O)2]·1/2H2O,
which, due to the low solubility in an aqueous medium, was modified to
increase its solubility for biological tests. The analysis of the spectra in
the medium infrared region (FTIR) for the complex [Ru(Cl)3(4-ampy)(H2O)2]·1/2H2O,
shows displacements of the bands observed at 1625 - 1566 cm﹣1 ν(C=C) e (C=N), indicating that coordination to the metallic center
occurred by this group. Band displacements were observed in the modified Ru
(III) complex, which suggests the presence of the 4-ampy ligand and the
coordination by the groups ν(C=C) and
(C=N) after the modification. In recent years, researchers worldwide have
concentrated on obtaining, developing, and modifying drugs used as
chemotherapeutic agents. The evaluation of the cell viability of the modified
Ru (III) compound demonstrated cytotoxic effects in the MCF-7 cell line (15.33%
± DP 2.7) but did not affect normal cells (PBMC), which reflects the potential
for possible applications.