TITLE:
Effect of intraperitoneal and intramuscular injection of killed aeromonas hydrophila on lymphocytes and serum proteins of common carp, cyprinus carpio
AUTHORS:
Rahim Peyghan, Gholamhosain H. Khadjeh, Naghmeh Mozarmnia, Maryam Dadar
KEYWORDS:
Aeromonas hydrophila; Globulins; Albumin, Lymphocytes; Vaccination
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.1 No.1,
April
22,
2010
ABSTRACT: The effects of injectable killed Aeromonas hydrophila on lymphocyte populations and on serum proteins of juvenile common carp, Cyprinus carpio, were examined. The fishes were injected either intraperitoneally or intramuscularly with killed A. hydrophila bacterin isolated from a diseased fish. 15 days after injection the lymphocyte count was significantly higher in the kidney of intraperitoneally bacteria injected fishes than in the normal saline injected fishes (p 0.05). In the intramuscular bacteria injected fishes in comparison with the normal saline group, the lymphocyte count was significantly higher only in the blood (p 0.05). In comparison between the serum protein parameters in different groups, in intraperitoneal injection of killed-bacteria, total albumin as well as the ratio of albumin/globulin was greater than the control group (p 0.05). In intramuscular injection, the normal saline-injected group, amount of α?-globulin was significantly higher than bacteria-injected group (p 0.05).