TITLE:
In Situ Detection of the p53-E6 and pRb-E7 Complex Formation in EC109 Cell by Proximity Ligation Assay
AUTHORS:
Yangjunqi Wang, Jintao Li, Fan Li, Shuying Li, Rugang Zhong
KEYWORDS:
HPV E6, HPV E7, p53, pRb, PLA
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.10 No.4,
April
28,
2019
ABSTRACT: Human papillomavirus is an important cancer factor in many cancers. E6E7 is the important viral oncogene and plays an important role. It is known that its function is to regulate p53 and pRb. In this paper, in situdetection was performed to determine the interaction between them; the research provides visual evidence of their interaction. Research methods: In situ PLA reaction was used to detect the relationship between two different protein. Results: The quantity of the HPV18E6 expression is much higher than the expression of HPV18E7 protein in HeLa and EC109 cells; the interaction between p53-E6 and pRb-E7 was clearly observed and this effect could be visualized by this method. Conclusion: Interaction of the HPV18E6 protein combined with p53 protein and HPV18E7 protein combined with pRb protein could be visualized in cell.