TITLE:
Molecular Evaluation of Garcinia kola Heckel Accessions Using RAPD Markers
AUTHORS:
Odunayo Joseph Olawuyi, Abeeb Abiodun Azeez
KEYWORDS:
Molecular Diversity, Genetic Relationships, RAPD, Genetic Improvement and Conservation
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.9 No.2,
April
9,
2019
ABSTRACT: The genetic relationships among twenty-five
accessions of Garcinia kola using six Random Amplified Polymorphic (RAPD) primers were evaluated in
this study. The highest volume of total genomic DNA (2218/μl) was recorded in
ON4 from Ikare, while the highest DNA concentration of 1.93 gl was found in OS3
from Ilesa. The highest Polymorphic Information Content (PIC) and gene
diversity of 93.77% and 0.94 respectively were revealed by primer OPO2 compared
to other primers. The dendogram generated from Unweighted Pair Group with Mean
Average (UPGMA) clustering delineated two groups, A and B, consisting of 21 and
4 accessions respectively. This study clearly showed the level of molecular
diversity in the accessions and the information provided could be utilized for
genetic improvement and conservation of Garcinia
kola.