TITLE:
A Study on the Aseptic Germination Method for Rosa rugose Seeds
AUTHORS:
Changli Fu, Shutang Xing, Dandan Zhao, Dekui Zang, Yuyuan Wei, Lanyong Zhao, Xiaoyan Yu
KEYWORDS:
Rosa rugose, Aseptic Germination, GA3 Treatment, Low Temperature Treatment
JOURNAL NAME:
Agricultural Sciences,
Vol.8 No.5,
May
27,
2017
ABSTRACT: In
order to find a method to break dormancy of Rosa rugose seeds and interspecific
hybrid seeds between Rosa rugose and Rosa hybrid quickly, and accelerate the breeding process of
interspecific hybridization between Rosa rugose and Rosa
hybrid, the influence of concentrated acid,
seed maturity, GA3 (gibberellin)
and low temperature (4°C) on seed germination of Rosa rugose from
Muping was researched under aseptic condition. The results showed that aseptic
germination can significantly shorten the germination time of Rosa rugose seeds
and raise its germination ratio. Before inoculation, concentrated acid
treatment greatly increased the germination rate and reduced the contamination
rate of the seeds. The higher the degree of maturity of seeds is, the lower the germination rate would be, and the
best time for seed to aseptic germination is 60 d after pollination. The addition of GA3 in 1/2MS medium could
promote seeds germination better, and when the concentration of GA3 was 0.15 mg/L, the seed germination ratio was the highest; the germination time
decreased and the seed germination ratio increased gradually as the treatment
time at 4°C lasted
longer.