TITLE:
Amino Acid Requirements of Gilthead Bream (Sparus aurata) Juveniles
AUTHORS:
M. M. Gaber, M. El-S. Salem, M. A. Zaki, A. M. Nour
KEYWORDS:
Amino Acid Requirements, Gilthead Bream, Whole Body, Tissues
JOURNAL NAME:
World Journal of Engineering and Technology,
Vol.4 No.3B,
August
25,
2016
ABSTRACT: In one trial 360 gilthead bream fingerlings received two different feed
mixtures containing two different levels of protein (diet A 400 and B 360 g?kg?1).
The average initial live weight was about 2.2 ± 0.1 g fish?1 in each
replicate of all treatments. Sixty fish per tank were stocked randomly in 6
fiberglass tanks (1 m3 each). Fish were fed with experimental diets
at rate 8% of the body weight daily. The Amino Acids (AA) content was analyzed
in the diets, whole fish and muscle at the end of the growth period (60 days).
The results indicated that at the end of experiment gilthead bream fingerlings
had reached an average live weight 18.5 and 16.2 g fish?1 for fish
fed diet A and B respectively. Also the increase of protein levels in the feed
to 40% caused a significant increase in the content of Indispensable Amino Acid
(IAA) and dispensable amino acid (as %v of whole fish and muscle of wet
weight). Two methods were subsequently used to estimate the quantitative IAA
requirements of gilthead bream fry based on the hypothesis that (a) the dietary
requirement pattern of IAA reflected the tissue pattern and (b) the rate of
daily deposition in the fish could be equated with the dietary requirement as
percentage of 100 g?1 diet. The quantitative IAA requirement (%) of
gilthead bream (Sparus aurata) diets was as follow: Lysine 2.27,
Methionine 1.09, Therionine 0.61, Leucine 2.4, Isoleucine 0.47, Histidine1.6,
Arginine 1.39, Phenylalanine 1.43, Valine 1.22 and Tryptophane 0.42.