TITLE:
Genetic diversity among Indian phytopathogenic isolates of Fusarium semitectum Berkeley and Ravenel
AUTHORS:
Avinash Ingle, Mahendra Rai
KEYWORDS:
Fusarium; Genetic Diversity; RAPD; Macroconidia; UPGMA
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.2 No.3,
May
23,
2011
ABSTRACT: We report total ten isolates of F. semitectum recovered from different hosts. Identity of these isolates was determined by morphological and cultural characteristics and confirmed by RAPD-PCR analysis using forty random primers. Morphologically all the ten isolates showed similarity, but based on RAPD-PCR analysis, these isolates can be categorized in three groups depending upon similarity co-efficient. Genetic similarity coefficients between pair wise isolates varied from 0.00 to 1.95 based on an unweighted paired group method of arithmetic average (UPGMA) cluster analysis. RAPD-PCR technique can be used as an important tool for the genetic differentiation among isolates of F. semitectum.