TITLE:
Influence of Substrate Feeding and Process Parameters on Production of Coenzyme Q10 Using Paracoccus denitrificans ATCC 19367 Mutant Strain P-87
AUTHORS:
Pradipta Tokdar, Prafull Ranadive, Rajendra Kshirsagar, Samanta Shekhar Khora, Sunil Kumar Deshmukh
KEYWORDS:
CoQ10, Paracoccus denitrificans, Cane Molasses, pHBA, Sucrose, DO, Process Optimization
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.5 No.12,
October
31,
2014
ABSTRACT: Coenzyme
Q10 (CoQ10), an important antioxidant molecule playing a major
role in electron transport chain, has been commercially produced by
fermentation process for the use in oral nutraceutical formulations.
Constructing the high-yielding CoQ10 producing strains is a
pre-requisite for cost-effective production. A superior mutant strain P-87
generated from Paracoccus denitrificans ATCC 19367, which showed 1.25-fold improvement in specific CoQ10 content higher than the wild type strain at shake flask level, was selected to
carry out the studies on CoQ10 yield improvement through fermenter
process optimization. In the course of study, initially the cane-molasses-based
medium and fed-batch fermentation strategy using pHBA in combination with
sucrose were standardized in shake flask using wild type strain. This strategy
was subsequently translated at 2 L laboratory fermenter while optimizing the
fermentation process parameters using improved mutant strain P-87. Under
optimized fermentation condition, mutant strain P-87 produced 49.85 mg/L of CoQ10 having specific content of 1.63 mg/g of DCW, which was 1.36 folds higher than
the specific CoQ10 content of wild-type strain under similar
optimized condition. The temperature and DO were found to be critical
parameters for CoQ10 production by mutant strain P-87. The optimum
temperature was found to be 32°C and the optimum DO concentration to be maintained
throughout the fermentation cycle was found to be 30% of air saturation.
Overall, a new cost-effective process has been established for the production
of CoQ10 using the cheaper substrate “cane molasses” and higher CoQ10 producing mutant strain P-87.