TITLE:
Histopathology and Immunohistochemistry Assessments of Acute Experimental Infection by Brucella melitensis in Bucks
AUTHORS:
Nurrul Shaqinah Nasruddin, Mazlina Mazlan, Mohd Zamri Saad, Hazilawati Hamzah, Jasni Sabri
KEYWORDS:
Histopathology, Immunohistochemistry, Brucella melitensis, Bucks
JOURNAL NAME:
Open Journal of Pathology,
Vol.4 No.2,
April
24,
2014
ABSTRACT:
Background: Brucellosis in male goats is characterized by arthritis, orchitis and
epididymitis, which may induce infertility. Nevertheless, these lesions were
categorized as chronic while acute lesions had not been described. This study
investigates the histopathological and immuno histochemistry reactions in
organs of bucks acutely infected by Brucella
melitensis. Results: Only testis and prepuce of acutely infected bucks
showed significantly severe histological lesions. Other internal organs had
mild to moderate lesions. However, positive immunohistochemistry stainings were
observed in organs except the bulbourethral gland. There was a significant
positive correlation between the distribution of B. melitensis and IHC intensity but no significant correlation
between the IHC intensity and histopathology lesions. Conclusion: The results
indicate that acute brucellosis did not lead to clinical presentation, although B. melitensis was well distributed in
various organs of bucks.