TITLE:
Analysis of Genetic Diversity among Wild and Cultivated Chickpea Genotypes Employing ISSR and RAPD Markers
AUTHORS:
Pramod Kumar Singh, Himanshu Sharma, Nidhi Srivastava, Sameer S. Bhagyawant
KEYWORDS:
Chickpea; Randomly Amplified Polymorphic DNA and Inter Simple Sequence Repeats; Genetic Diversity
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.5,
March
14,
2014
ABSTRACT: Chickpea (Cicer
airetinum L.) is an important and
most preferred food legume in many parts of the world especially in the Indian
sub-continent. Molecular analysis of chickpea using DNA technology has been
carried out to identify the diverse genetic base of the cultivars for selected
preferential introductions as efficient edible elements. A few of these genetic
stocks have been documented here to tap their genetic diversity. The status
shows that the level of polymorphism
in this species is low. Using PCR based markers, marker assisted selection of
polymorphy is one of the established techniques. Here, this procedure has been
employed to expedite gene/QTL pyramiding in the chickpea. The study presented
here includes analysis of 12 germplasms of chickpea. Standard CTAB method has
been performed, with certain modifications, to get better intensity and resolution
of DNA bands. Extracted DNA, amplified with 41 RAPD and 21 ISSR primers are
thereby tested to determine genetic diversity. The presentation discusses the
results of chickpea germplasm diversity on the basis of these observations.