TITLE:
The Expressions of P53, MDM2 in Trophoblasts of Spontaneous Abortion Mouse Model and the Relevant Researches
AUTHORS:
Chunlan Zhou, Qian Wang, Menghuan Zeng, Liping Cheng, Xiaoyun Xie
KEYWORDS:
p53; MDM2; VEGF; HIF-la; Spontaneous Abortion
JOURNAL NAME:
Engineering,
Vol.5 No.10B,
December
17,
2013
ABSTRACT:
Objective: To explore the mRNA expression of the related
genes of p53, MDM2, vascular endothelial growth factor (VEGF) and hypoxia
inducible transcription factors-1 a (HIF-la) in villous samples of spontaneous
abortion mouse models and normal pregnancy models, and to discuss the effect of
p53, MDM2 on the growth of villous trophoblast cells. Methods: The abortion-prone
CBAXDBA/2 matings were established as the model of spontaneous abortion and the
non-abortion-prone CBAXBALB/c matings as the model of normal pregnancy. Applied
q Real-time PCR method to detect the mRNA expression levels of p53, MDM2, VEGF
and HIF-la in villous samples of spontaneous abortion mouse models and normal
pregnancy models. Results: The relationship of the mRNA expression level of p53,
MDM2, VEGF and HIF-la in vinous samples of spontaneous abortion mouse models:
in the villous samples of spontaneous abortion mouse models, the expression of
p53 was positively correlated with the expression of MDM2, HIF-la (r = 0.35; r
= 0.63), and the relationship was significant (P = 0.01; P