TITLE:
Functional analysis of OAT gene in Aspergillus Niger
AUTHORS:
Chuang Zhou, Xihong He, Hao Liu
KEYWORDS:
Aspergillus Niger; Oxaloacetate Transporter; Organic Acids; Glycerol Formation
JOURNAL NAME:
Engineering,
Vol.4 No.10B,
October
21,
2013
ABSTRACT:
Mitochondrial oxaloacetate
transporter protein encoded by OAT gene transports oxaloacetate from cytoplasm
into mitochondria. To investigate the primary effects of OAT gene on relative
metabolism in Aspergillus niger, a oat-deleted
mutant was derived from wild-type A.
niger ATCC1015 using the double-crossover chromosome replacement technique.
Then batch fermentation was performed to evaluate the mutant. Compared with the
wild type, the mutant showed lower organic acids production, with the experimental
data that the production of pyruvate and 2-ketoglutarate decreased by 31.6% and
35.7%, respectively, and by contrast, the mutant showed higher glycerol
formation. The results suggest that OAT gene plays significant roles on metabolism
in A. niger.