L. Thomas, J. M. Wallace, R. P. Aitken, J. G. Mercer, P. Trayhurn, and N. Hoggard, “Circulating leptin during ovine pregnancy in relation to maternal nutrition, body composition and pregnancy outcome,” J. Endocrinol., vol. 169, pp. 465–476, Jun. 2001.

has been cited by the following article:

• TITLE: Leptin Receptor in Ram Epididymal Spermatozoa

  AUTHORS: Mohammad Roostaei-Ali Mehr, Reza Rajabi-Toustani, Rasool Motamedi-Mojdehi

  KEYWORDS: Epididymal Spermatozoa; mRNA; Ob-R; Ob-Rb

  JOURNAL NAME: Engineering, Vol.4 No.10B, October 21, 2013

  ABSTRACT: This experiment was designed to investigate leptin receptors (Ob-R and Ob-Rb) mRNA in ram epididymal spermatozoa by RT-PCR. Ten testes were obtained from abattoir and epididymal spermatozoa recovery was performed. To purify spermatozoa, motile sperm were isolated by the swim-up procedure. Total RNA was isolated from epididymal spermatozoa and placental cotyledon, as a positive control, and then they were purified. Specific bands (98 and 308 bp) for Ob-R and Ob-Rb were detected after RT-PCR in both epididymal spermatozoa and placental cotyledon. We may conclude that Ob-R and Ob-Rb mRNA are present in ram epididymal spermatozoa and leptin perhaps exerts physiological effects, as already demonstrated in human.